agricultural mixtures

专利摘要:
This document describes methods and systems for handling barium recyclable biological streams in order to produce agricultural mixtures. The resulting agricultural mixtures can be used to improve the yield of the crop or as a feed for animals. Managing sources of variable biological recyclable currents can provide agricultural mixtures of controlled properties.
公开号:BR112020002523A2
申请号:R112020002523-2
申请日:2018-08-13
公开日:2020-08-04
发明作者:Daniel Morash；Mark LeJeune；Steve Zicari
申请人:California Safe Soil, LLC；
IPC主号:

专利说明:

[0001] [0001] The present invention relates to methods and systems for handling recyclable biological streams and for mixing recyclable streams and recyclable minerals to obtain agricultural mixtures and the compositions produced by them. RELATED ORDER DATA
[0002] [0002] This application claims priority to US Provisional Patent Application serial number 62 / 544,579, filed on August 11, 2017, the content of which is incorporated into this document as a reference in its entirety. BACKGROUND
[0003] [0003] The following includes information that may be useful in understanding the present invention. It is not an admission that any of the information, publications or documents mentioned in this document in a specific or implicit manner constitute prior art, or are essential to the inventions currently described or claimed. All publications and patents mentioned in this document are incorporated into this document, by reference, in their entirety.
[0004] [0004] In the United States of America, food production uses approximately 50% of the land and uses 80% of the total fresh water consumed. About 40% of total food production, however, is wasted (Gunders, D., “Wasted: How America Is Losing Up to 40 Percent of Its Food from Farm to Fork Landfill,” NRDC Issue Paper IP: 12-06 -B (August 2012)), which equates to US $ 200 billion each year. While maximizing efficiency in the US food system from farm to fork attracted a lot of public attention, the productive uses of food waste are underdeveloped.
[0005] [0005] The organic matter in the soil is negatively impacted by cultivation and / or prolonged periods without vegetation cover, which can decrease the organic matter content below natural or virgin levels for a given location. Depletion of soil organic matter is a serious threat to limited agricultural resources. Global food production depends on fertile soils (Lai, et al., "Climate Strategic Soil Management", Challenges, 5: 43-74 (2014); Blanco-Canqui, et al., "Principles of Soil Conservation and Management," Springer , Netherlands (2008)), which are a finite resource, requiring protection and efficient use by farmers who produce food sources for animal and human consumption. Typical animal feed comes from corn, hay, alfalfa, soy, rice, sorghum, wheat and oats. Animal feed is usually supplemented with peanuts, soybeans, corn gluten and cottonseed to increase the protein content of the feed. SUMMARY
[0006] [0006] The inventions described and claimed in this document have many attributes and aspects, including, among others, those established or described or mentioned in this Brief Summary. It is not intended to be comprehensive, and the inventions described and claimed in this document are not limited to, or by the characteristics or modalities identified in this Brief Summary, which is included for illustrative purposes only and not for restriction.
[0007] [0007] The ability to process and manage the processing of one or more recyclable biological streams and to combine processed products to produce agricultural additives offers numerous benefits.
[0008] [0008] In one aspect, this disclosure refers to a process for producing an agricultural mixture, from a selected recyclable biological stream including the steps of: (a) providing a recyclable biological stream using a collection system; (b) grinding the recyclable biological stream using a first grinder and optionally a second grinder to produce a ground biological slurry; (c) adding one or more selected enzymes to said ground biological paste; (d) increasing the temperature of the organic pulp ground from room temperature to a temperature between about 35ºC and about 60ºC and incubating the organic pulp under constant agitation and shearing at two or more temperatures between about 35ºC and about 60ºC, thus producing an incubated biological paste comprising incubated biological particles and an incubated biological hydrolyzate comprising an oil phase and an aqueous phase; (e) pasteurization of the first incubated paste to exterminate pathogens; (f) optional separation of the first incubated hydrolyzate into a first incubated biological hydrolyzate and first biological particles incubated using one or more separation methods based on size; (g) optional reduction of the fat content of the first pasteurized incubated hydrolyzate optionally by centrifugation to form a centrifuged biological hydrolyzate and a centrifuged oil; (h) alternatively, where when steps (f) and (g) are not performed, the method optionally comprises steps (A) to (C): (A) drying the first incubated and pasteurized biological paste to form a solid, dry biological paste; (B) grinding the solid biological slurry to form a dry biological slurry powder or granulating the dry solid slurry to form microspheres of dry biological slurry; (C) optional mixing of the dry, organic biological pulp or microsphere of dry biological pulp, with a recyclable stream of carbohydrates to form Animal Food (I); or where where step (f) and step (g) are performed, the method optionally further comprises steps (D) and (E):
[0009] [0009] In some aspects of this disclosure, the stabilized aqueous hydrolyzate can also be concentrated and / or mixed with an additive.
[0010] [0010] In some aspects of this disclosure, it was found that Animal Food (I), in addition to being produced from sustainable biological recyclables, surprisingly results in a higher conversion rate in mass of feed to animal weight compared to a standard feed product, with an observed increase in animal weight when used as food compared to control. The inventors have also surprisingly found that recycling processed foods using the methods described in this document into animal feed administered to them results in healthier animals (for example, exhibiting reduced diarrhea and / or lower glucose levels) and growth faster compared to conventional animal diets.
[0011] [0011] In one aspect, the concentration of the liquid hydrolyzate is carried out using filtration or evaporation. In some respects, steps (e), (f) and (g), described above, can be performed in any order.
[0012] [0012] In some respects, when the pasteurized fat content was incubated for the first time, the hydrolyzate is reduced by centrifugation, the centrifuged oil or the alternatively centrifuged biological hydrolyzate is added to the biological paste before drying to modulate the fat content in the resulting mixture. In some ways, the centrifuged oil is further separated into a stream of unusable oil for food and a stream of oil usable in food. The oil stream usable in food can be used as Animal Food (III), and the oil stream usable in food can be used in the production of biofuels. When the stream of unusable oil from food is used in the production of biofuels, the stream of unusable oil from food can be refined by distillation into fuels. In some aspects, the centrifuged oil comprises fatty acids, triglycerides, triglycerol and / or fatty acid esters.
[0013] [0013] In some respects, when the first incubated hydrolyzate is separated into a first incubated biological hydrolyzate and the first biological particles incubated using one or more size-based separation methods, the size-based separation method comprises the use of a screen, mesh or separator to remove undigested material, for example, a coarse screen. In some aspects of this disclosure, a second screen, mesh or separator can be used alone or in combination with the first separation method, where the second separation method is used to remove particles too large for adjustment through drip lines or other liquid transport pipes, for example, a fine sieve or both. The separation steps can, in some respects, be performed using screens as vibrating screens.
[0014] [0014] In some respects, an anti-caking and / or antioxidant agent is added to any of the animal feeds described in this document before or in the final stage of the process. In some ways, an anti-caking and / or antioxidant agent is added to the dry, solid biological paste.
[0015] [0015] In some aspects, the stabilization step (D) comprises the addition of a stabilizer selected from: inorganic acid, organic acid, organic preservative and inorganic preservative.
[0016] [0016] In some aspects, the emulsification step (E) comprises the use of a high shear mixer.
[0017] [0017] In some respects, the first biological particles incubated are dehydrated. In some respects, the first incubated biological particles are dehydrated using a screw press, belt filter or hydraulic press to form separate dehydrated biological particles and a recycled liquid fraction. In some aspect, the recycled liquid fraction can be added to any of the liquid compositions described in this document. The dehydrated biological particles can be used as a compound, source of biofuel or as Animal Food (IV). In some ways, the compost can be composted with a mineral. In some ways, the mineral can be extracted from basalt. Basalt compost can be used as a high mineral content fertilizer. In some respects, the basalt compound can be combined with the agricultural additives described in this document, to be used as a fertilizer.
[0018] [0018] In some respects, the hydrolyzate emulsified from a batch of production can be stored and mixed in one or more storage tanks with one or more circulation pumps to form an agricultural mixture.
[0019] [0019] In some respects, the process may also include processing a second or more recyclable biological streams using the methods described for the first recyclable stream. Products formed from the second or more recyclable biological streams can be added to the products of the first recyclable stream at any point in the process.
[0020] [0020] In one aspect, the production method of agricultural mixtures comprises grinding a first recyclable biological stream selected to form a first ground organic pulp, heating and incubating the first ground organic pulp with one or more enzymes with constant agitation and shearing and pasteurization of the first incubated ground organic pulp to produce a pasteurized first ground organic pulp for use in an agricultural mixture.
[0021] [0021] In one aspect, this disclosure describes methods and systems for processing two or more recyclable biological streams selected to form incubated biological hydrolysates from each stream and combining the incubated biological hydrolysates to obtain agricultural mix compositions, for example, biological hydrolysates combined, concentrated, dried cakes or biological particles incubated and combined. Agricultural mixtures are useful for supplying food and minerals to ground plants and microbes and / or animals. This disclosure also describes the mixtures and hydrolysates obtained from these processes and systems. The methods of this disclosure allow the recycling of recyclable biological currents that would otherwise be selected in landfills or other similar facilities, wasting the said recyclable biological currents.
[0022] [0022] In one aspect, the incubated biological hydrolyzate may comprise one or more phases. In some respects, the incubated biological hydrolyzate may comprise an aqueous phase and an oil phase. In some aspects, the oil phase of the incubated biological hydrolyzate may also comprise fatty acids, biodiesel oils and / or edible oils. The aqueous phase, the oil phase and, optionally, the biological particles can be separated by a three-phase separator by the processes described in this document. In some respects, the three-phase separator is a tri-vessel centrifuge. In some ways, the tricanter centrifuge is a Flottwegg separator (Germany). In some ways, the three-phase centrifugal separator is a peony centrifuge (China). In some respects, the three-phase centrifugal separator is an Alfa Laval centrifuge (Sweden). In some respects, the incubated biological hydrolyzate can be separated using a hydrocyclone to separate particles from liquids. The hydrocyclone can be a sand separator from Netafim (USA) or a John Deer F 1000 sand separator (Deer, USA).
[0023] [0023] When used as fertilizer and for soil correction, the agricultural mixtures in this disclosure provide higher crop yields, for example, by providing food to plants in the form of nutrients and increasing the organic matter in the soil and supporting the growth of beneficial organisms in the soil. ground. In some respects, the agricultural mixtures in this disclosure increase crop productivity, in addition to reducing the use of nitrate or ammonia based fertilizers, which reduces the flow of nitrate into lakes and streams and reduces potent greenhouse effect emissions. (according to EPA, N2O or nitrous oxide, emitted with nitrate or ammonia-based fertilizers, is 300 times more potent than a greenhouse gas than carbon dioxide (IPCC (2007) Climate change 2007: The Physical Science Basis. S. Solomon et al., Eds. Cambridge University Press, Cambridge,, United Kingdom)). Therefore, the use of agricultural mixtures in this disclosure to replace some or all nitrate or ammonia based fertilizers can mitigate the problems associated with the use of chemical fertilizers, such as nitrate runoff, GHG emissions and / or reduction of organic matter in ground. In addition, the agricultural mixtures in this disclosure also increase the vigor of the plants and the growth of the root system, increasing the absorption of nitrates by the plants and, thus, further reducing the flow of nitrate or ammonia-based fertilizers in the water supply and increasing the efficiency of water and fertilizer use for the farmer. {See, for example, Dara et al., Evaluating a Recycled Food Waste-Based Liquid Compost in Conventional California Strawberries, Agricultural Research & Technology Open Access Journal vol. 12 (2) (October 2017), 1-3)
[0024] [0024] As used in this document, the term "crop yield" refers to a measure of the amount of a crop that has been harvested per unit of land area. Harvest yield can also refer to the actual seed generation of the plant. The unit by which a crop's yield is measured is kilograms per hectare, bushels per acre or tons per acre.
[0025] [0025] Efficiency in the use of water is a matter of growing concern, due to the impact of drought and climate change. The agricultural mixtures in this disclosure can also increase water retention through the accumulation of organic matter in the soil and improve the ground slope (including the formation and stability of aggregated soil particles, moisture content, degree of aeration, water infiltration rate , and drainage). In addition, the agricultural mixtures in this disclosure produce high crop yields at relatively lower costs, improve crop quality and promote crop resistance to pests, diseases and plant stresses (such as salt, poor soil, heat or drought).
[0026] [0026] The recyclable biological currents selected are a waste of resources and a major source of greenhouse gas emissions (for example, carbon in the form of CO2 (carbon dioxide) or CH4 (methane) which, according to the EPA , is 23 times more potent than a greenhouse gas like carbon dioxide). Recyclable biological currents can start to decompose quickly, creating a public safety and nuisance problem, making it difficult, if not impossible, to use valuable recyclable biological currents. This disclosure features the use of various recyclable biological streams to make agricultural mixes and animal feed, which reduce greenhouse gas emissions associated with the decomposition of biological waste. In some respects, the processed recyclable biological stream produces less methane emissions than unprocessed recyclable biological stream. In another aspect, agricultural mixtures produced by the methods of this disclosure facilitate the growth of beneficial microbial populations in the soil. The increase in microbial activity increases carbon sequestration in the soil, thus improving the sustainability of agricultural practices. The nutrients in the agricultural mixtures described in this document stimulate microbial life in the soil. The detritus of ground microbial life is the basis for long-term carbon sequestration (Kallenbach, C. et al., Nature Comm., 7: 13630 (2016); Lehmann, J., Nature, 528: 60-69 (2015)).
[0027] [0027] By recycling recyclable biological currents that would otherwise rot and ferment, releasing prodigious amounts of greenhouse gases, in addition to toxic liquids and gases (C2H5OH or ethanol, a plant pathogen and H2S (hydrogen sulfide, a toxic gas) and other by-products of related effluents from rot and fermentation, the methods of this disclosure fully utilize the nutritional content of recyclable biological currents and dramatically reduce residual organic matter and its risk resulting from sheltered pathogens, while providing benefits significant to soil or animal feed.The methods described in this document avoid the possibility of contamination, preventing the introduction of disease-causing pathogens (including or excluding salmonella, E. coli and listeria, which may be present in the recyclable biological input stream ) ground when used as fertilizer (Pandey, P. et al., J. Cl eaner Prod., 1-9 (2015)).
[0028] [0028] In some aspects of this disclosure, the collection system for this disclosure captures the nutritional value of the recyclable biological stream using a system (which minimizes the final selected waste) that does not allow recyclable biological streams to rot (non-putrescent recyclable biological streams) . Putrescence can be measured by odor tests or by GC-MS (gas chromatography) analysis of the space above the test sample or by a portable odor monitor (for example, Kanomax OMX-TPM or Shinyei portable odor meter OMX-SRM). In some aspects of this disclosure, the supermarket team separates some forms of recyclable biological currents
[0029] [0029] In some aspects of this disclosure, rendering facilities may provide poultry feathers, beaks and feet of poultry (recyclable poultry chain) and / or bone meal. In some ways, fish processing plants can supply fish products as a recyclable fish chain. Fish recyclables can include or exclude: skin, offal, fish heads, fish tails, fish hydrolyzate and carcasses (fish bones). Fish recyclables can increase the relative amount of organic nitrogen in the hydrolyzate. Ethanol plants can produce grains from the distiller, which when added to the processes described in this document can increase the carbohydrate content in the hydrolyzate.
[0030] [0030] In some respects, recyclable biological streams may include or exclude any of the previous recyclable biological streams.
[0031] [0031] In some respects, the recyclable biological stream may include or exclude selected fruits, nuts or vegetables, for example, selected nuts or seeds of cucurbits.
[0032] [0032] In some respects, the agricultural mixtures described in this document can be further combined with organic fertilizers to produce a synergistic effect of organic fertilizers and the agricultural mixtures described in this document to improve crop yield and organic soil content . Organic fertilizers may include or exclude bone meal, blood meal, feather meal or manure, for example, chicken manure, bird guano, biosolids (treated solids from wastewater treatment plants), cattle manure, compost green waste or combinations thereof. It has surprisingly been found that the processed agricultural mixtures described in this document, when mixed with an organic fertilizer, provide granulation of the combined product and / or result in faster decomposition of the organic fertilizer into nutrients to improve plant growth rates and / or crops and crop yields.
[0033] [0033] In some aspects of this disclosure, the recyclable biological chain is placed in separate and / or airtight bags and / or crates that keep recyclable biological chains cold that supermarkets, food processors, wholesalers, bakeries or other sellers or manufacturers do not. offer more sales. For example, non-putrescible food recyclables, selected vegetables or bagasse can be stored and transported in separate and / or airtight bags and / or crates.
[0034] [0034] In some respects, the separate bags and / or crates used to collect food may be double-walled.
[0035] [0035] In one aspect this disclosure presents a method that is described to produce an agricultural mixture, from one or more recyclable and selected biological streams, comprising the steps of: (a) provision of a selected recyclable biological stream using a system collection; (b) grinding the selected recyclable biological stream using a first grinder and optionally a second grinder to produce a first ground biological paste; (c) adding to said first ground biological paste one or more selected enzymes; (d) increasing the temperature of the first biological pulp ground from room temperature to a temperature between about 35ºC and about 60ºC and incubating the first organic ground pulp with constant agitation and shear at two or more temperatures between about 35ºC and about 60ºC, thus producing a first incubated biological paste comprising incubated biological particles and a first incubated biological hydrolyzate; (e) pasteurization of the first biological paste incubated to exterminate pathogens; and (f) separating the first incubated biological paste into a first incubated biological hydrolyzate and incubated biological particles.
[0036] [0036] In some aspects, the step of adding one or more selected enzymes to the first ground organic paste is carried out before or during the step of increasing the temperature of the first ground organic paste from room temperature to a temperature between about 35ºC and about 60ºC and incubation of the first ground biological paste. In some respects, one or more selected enzymes can be added after the first ground biological paste is heated to a temperature between about 35ºC and about 60ºC. In some respects, one or more selected enzymes can be added in powder or liquid form. In some aspects, the liquid form of one or more selected enzymes can be preheated and / or accelerated with the co-addition of one or more cofactors. In some respects, one or more selected enzymes is added with one or more cofactors. In some ways, the cofactor may include or exclude metal cations and coenzymes. Metal cations can include or exclude: cupric, ferrous, ferric, catalase, magnesium, manganese, molybdenum, nickel and zinc, coenzymes can include or exclude vitamins and vitamin derivatives of: thiamine pyrophosphate, thiamine, NAD + and NADP +, niacin , pyridoxal phosphate, pyridoxine, methylcobalamin, vitamin B12, cobalamin, biotin, coenzyme a, pantothenic acid, tetrahydrofolic acid, folic acid, menaquinone, vitamin K, ascorbic acid, flavin mononucleotide, riboflavin and F420 coenzyme.
[0037] [0037] In some respects, the first temperature of the first incubated ground biological paste can be 35, 35.5, 36.1, 36.6, 37.2, 38.3, 38.8, 39.4, 40 , 40.5, 41.1, 41.6, 42.2, 42.7, 43.3, 43.8, 44.4, 45, 45.5, 46.1, 46.6, 47.20 , 47.70, 48.3, 48.8, 49.4, 50, 50.5, 51.1, 51.6, 52.2, 52.7, 53.3, 53.8, 54.4 , 55, 55.5, 56.1, 56.6, 57.2, 57.7,
[0038] [0038] In some respects, the method in (h) (A) (which may or may not include steps (f) or (g)), may use a drum dryer (such as can be manufactured by Andritz, Drum Drying Systems, Buflovak, GL&V or Phoenix Drum Drying), a spray dryer (as can be manufactured by Pulse Combustion Systems or GEA), extrusion dryers (as they can be manufactured by Diamond America or Coperion) or a rotary kiln (such as as can be manufactured by Feeco) to produce a dry hydrolyzate. In some respects, in the method in (g) (B), the dry hydrolyzate can be ground into powder using a standard fitz mill, or granulated using a common granulator to form dry hydrolyzate granules for animal feed. The powder or granules in (g) (B) may or may not include the addition of stabilizing and / or anti-caking agents. In some respects, in the method described in (g) (C), the animal feed can be mixed with other ingredients of the animal feed, to be customized for specific applications.
[0039] [0039] In some respects, step (f) results in the reduction of the number of particles in the liquid biological hydrolyzate. In some respects, step (f) is performed using selective size separation methods. In some respects, selective size separation is performed using a centrifugal separation system. In some respects, selective size separation methods use a reusable filter or mesh. In some respects, selective size separation is carried out by serial filtration through a coarse sieve followed some time later by filtration through a fine sieve. In some ways, the filter or mesh is made of metal, plastic, glass or ceramic.
[0040] [0040] In some respects, the first incubated biological hydrolyzate comprises one or more phases. The first incubated biological hydrolyzate can comprise an oil phase, a particulate phase and an aqueous phase. In some respects, the method in (g) of separation is carried out using a three-phase separator. In some ways, the three-phase separator is a centrifugal separator. The three-phase separator can separate all or part of a heavy liquid phase, light and solid liquid, due to its different densities and mutual insolubility. The solid phase differentially settles into a centrifugal force field or gravity force field, which causes the deposition of solid particles in the liquid. In some ways, the three-phase centrifugal separator is, for example, a Flottweg separator. In some respects, the three-phase centrifugal separator is, for example, a peony centrifuge. The three-phase separator operates at 1,000 - 7,000 RPM and processes 5 to 50 gallons per minute. In some ways, the three-phase separator processes 5 to 50 gallons per minute. In some ways, the three-phase separator processes 15 gallons per minute. In some aspects, several three-phase separators can be placed in series or in parallel. When multiple three-phase separators are placed in parallel, the first incubated biological hydrolyzate can be processed more quickly with a shorter process time per separator than if the first incubated biological hydrolyzate were processed with a single three-phase separator. In some respects, the three-phase centrifugal separator is, for example, an Alfa Laval centrifuge. In some respects, the incubated biological hydrolyzate can be separated using a hydrocyclone to separate particles from liquids. The hydrocyclone can be a sand separator from Netafim (USA) or a John Deer F 1000 sand separator (Deer, USA).
[0041] [0041] In some aspects, the method also comprises (h) (D) stabilization and preservation of the incubated biological hydrolyzate, using a stabilizer selected from: inorganic acid, organic acid, organic preservative, inorganic preservative. The stabilization and preservation step can take place before or after the separation step (f). In some respects, the method comprises (h) (E) emulsifying the incubated and stabilized biological hydrolyzate using an ultra-high shear mixer and / or organic or inorganic emulsifiers to produce a stabilized emulsified hydrolyzate. In some aspects, the emulsification step may include the addition of organic and / or inorganic dispersants to act as surface active ingredients in the emulsified and stabilized hydrolyzate. In some respects, the method comprises (h) (E) (ii) mixing the stabilized emulsified hydrolyzate in one or more storage tanks using one or more circulation pumps with other liquid fertilizing ingredients that may include or exclude vitamins, pesticides, tracking inorganic minerals, wood ash, plaster salts, Epsom salts, earthworm castings, dyes, fragrances and viscosity modifiers.
[0042] [0042] In some respects, the method further comprises (h) (E) (i) concentrating the liquid hydrolyzate through vibratory filtration equipment (such as that which can be manufactured by New Logic) or vacuum evaporation equipment (such as manufactured by Buflovak or Vobis). In some aspects, the method further comprises (h) (E) (ii), mixing the concentrated liquid hydrolyzate with other liquid fertilizing ingredients or ingredients for animal feed.
[0043] [0043] In some aspects of this disclosure, the method further comprises further processing the biological particles sieved from step (f), using a separation method, for example, using a screw press, belt press or hydraulic press to produce a optionally recyclable liquid fraction, and a step comprising the dehydrated biological particle fraction. The fraction of dehydrated biological particles can be used as a raw material for green compost, basalt compost, other compounds, as well as biofuel or animal feed in an agricultural mixture. The liquid fraction can, in some aspects, be added to the biological hydrolyzate from the biological paste.
[0044] [0044] In some aspects of this disclosure, the finished product of the stabilized emulsified hydrolyzate is homogeneous. In some respects, homogeneity can be quantified by viscosity measurements using a rotation viscometer (for example, Thermo Scientific ™ HAAKE ™ Viscotester). In some respects, the viscosity of three samples of the finished product may be within an experimental error of each other. In some aspects, biological particles comprise bone, cellulose, solidified or semi-solid fats, nutshells, fish scales, teeth, inorganic minerals, species containing keratin or combinations thereof. In some respects, species containing keratin are selected from: beaks, feathers, claws or hair. In some respects, the incubated hydrolyzate is separated from the incubated particles using one or more sieves. In some respects, the incubated hydrolyzate is separated from the incubated particles by centrifugation, sedimentation, use of a hydrocyclone, a rotospiral drum screen or a horizontal belt filter. In some ways, the separated biological particles can be processed as a second recyclable biological stream.
[0045] [0045] In some respects, the first grinder and the second grinder are not in fluid communication with each other. In some aspects, the first grinder and the second grinder are in fluid communication. In some respects, the first grinder is not in fluid communication with the incubation vessel.
[0046] [0046] In some respects, steps (a) - (b) can be performed in a different location, physically separate from the place where steps (c) - (h) are performed. In some respects, the two different sites are more than 3.04 m, 30.48 m, 304.8 m, 1.60 km, 8.04 km, 16.09 km, 160.93 km from each other. In some respects, steps (a) - (b) can be performed on a mobile platform.
[0047] [0047] In some aspects of this disclosure, steps (a) - (e) and, optionally, steps (f), (g) and / or (h), are repeated with at least one second, third or more streams selected recyclables, thus producing at least a second stream of incubated biological particles and at least a second incubated biological hydrolyzate. The combination of the first incubated hydrolyzate with at least the second incubated hydrolyzate produces an agricultural mixture. The combination of the first incubated particles with at least the second incubated particles produces an agricultural mixture useful as a liquid plant fertilizer, concentrated plant fertilizer or animal feed or dry animal feed. Therefore, in some aspects of this disclosure, the process for producing an agricultural mixture from selected recyclable biological streams further comprises (i) the step of incorporating the enzymatically digested hydrolyzates combined from more than one recyclable stream through the steps (a ) - h) to produce agricultural additives.
[0048] [0048] In one aspect, this disclosure presents a method for producing an agricultural mixture from a plurality of selected recyclable biological streams, comprising the steps of:
[0049] [0049] Each of the steps mentioned above can characterize any of the modalities of the step presented in this disclosure, and the method can comprise the processing of additional recyclable chains in addition to the first and second recyclable chains.
[0050] [0050] In one aspect, this disclosure presents a method for producing an agricultural mixture from a plurality of selected biological recyclables, comprising the steps of: (a) providing a first recyclable biological stream using a collection system;
[0051] [0051] Each of the steps mentioned above can present any of the modalities of the step presented in this disclosure, and the method can comprise the processing of additional recyclable chains.
[0052] [0052] In this regard, this disclosure presents, for example, a process for producing an agricultural mixture from a plurality of selected recyclable biological streams, comprising the steps of: (a) providing a first recyclable biological stream using a system of collect; (b) grinding the first recyclable biological stream using a first grinder and optionally a second grinder to produce a first ground biological paste; (c) adding to said first ground biological paste one or more selected enzymes;
[0053] [0053] Each of the steps mentioned above can present any of the modalities of the step presented in this disclosure, and the method can comprise the processing of additional recyclable chains.
[0054] [0054] In one aspect, any of the processes for producing an agricultural mixture from a plurality of selected recyclable biological streams described above further includes the step (n) of adding a stabilizer while the hydrolyzate is mixed. The addition of the stabilizer can occur before the separation of the first or second ground biological paste, before mixing the first incubated biological hydrolyzate with the second incubated hydrolyzate, while mixing the incubated biological hydrolyzate with the second incubated hydrolyzate or after mixing the first incubated hydrolyzate with the second incubated hydrolyzate. In one aspect, the stabilizer is an acid or preservative. The acid can be an organic acid or inorganic acid. The preservative can be any preservative described in this document, including a preservative suitable for the labeling of organic products.
[0055] [0055] As a representative example, in one aspect, the process for producing an agricultural mixture from a plurality of selected recyclable biological streams may comprise the steps of: (a) providing a first recyclable biological stream using a collection system ; (b) grinding the first recyclable biological stream using a first grinder and optionally a second grinder to produce a first ground biological paste; (c) adding to said first ground biological paste one or more selected enzymes; (d) increasing the temperature of the first biological pulp ground from room temperature to a temperature between about 35ºC and about 60ºC and incubating the first organic pulp ground under constant agitation and shear at two or more temperatures between about 35ºC and about 60ºC, thus producing a first incubated biological paste comprising incubated biological particles and a first incubated biological hydrolyzate; (e) pasteurization of the first biological paste to exterminate pathogens; (f) separating the first biological paste into a first incubated biological hydrolyzate and the first incubated biological particles; (g) provision of at least a second recyclable biological stream using a collection system; (h) grinding the second recyclable biological stream using a first grinder and optionally a second grinder to produce a second ground biological pulp; (i) adding to said second ground biological paste one or more selected enzymes; (j) increasing the temperature of the second organic pulp ground from room temperature to a temperature between about 35ºC and about 60ºC and incubating the second organic pulp ground under constant agitation and shear at two or more temperatures between about 35ºC and about 60ºC, thus producing a second incubated biological paste comprising incubated biological particles and a second incubated biological hydrolyzate; (k) pasteurization of the second ground biological paste incubated to exterminate pathogens; (1) separation of the second incubated ground biological paste into a second incubated biological hydrolyzate and second incubated biological particles; (m) mixing the first incubated biological hydrolyzate with the second incubated biological hydrolyzate to form an agricultural mixture; and (n) adding a stabilizer while the hydrolysates are mixed.
[0056] [0056] Each of the steps mentioned above can present any of the modalities of the step presented in this disclosure, and the method can comprise the processing of additional recyclable chains.
[0057] [0057] In some respects, any of the processes for producing an agricultural mixture from a plurality of selected recyclable biological streams described in this document further includes the steps of: (o) dehydrating the incubated hydrolyzate mixture to form an agricultural mixture dry.
[0058] [0058] In some respects, the method for manipulating a process to produce an agricultural mixture from a plurality of selected recyclable biological streams described in this document may further include the steps of: (p) mixing the stabilized and dry emulsified hydrolyzate with the biological particles incubated to form an agricultural mixture.
[0059] [0059] In some aspects, any of the processes described in this document may also include the steps of: (q) provision of a selected third recyclable biological stream; (r) grinding the selected third recyclable biological stream using a first grinder and optionally a second grinder to produce a third ground biological pulp; (s) adding to said ground biological paste one or more selected enzymes; (t) increasing the temperature of the third organic pulp ground from room temperature to a temperature between about 35ºC and about 60ºC and incubating the third organic pulp ground in constant agitation and shearing at two or more temperatures between about 35ºC and about 60ºC, thus producing a third incubated biological paste comprising incubated biological particles and a third incubated biological hydrolyzate; (u) separating the third incubated ground biological paste into a third incubated biological hydrolyzate and third incubated biological particles using a coarse sieve and a fine sieve; (v) pasteurization of the third biological hydrolyzate incubated to exterminate pathogens; (w) mixing the third incubated biological hydrolyzate with the mixture of the first and second biological hydrolysates to form an agricultural mixture.
[0060] [0060] In some respects, the biological hydrolysates described in this document may comprise one or more liquid phases. In some aspects, the liquid phases may comprise an aqueous phase and an oil phase. In some respects, the oil phase can further be separated into a usable edible oil composition and an unusable oil composition as food. As used herein, the term "oil not usable as food" is an undistilled oil that is not suitable for animal feed. As used herein, the term "usable edible oil" is a non-distilled oil that can be incorporated into animal feed (or as a raw material for the production of biodiesel, depending on market conditions). In some ways, usable edible oil is a low-titration oil from a plant or walnut oil. In some respects, the usable edible oil composition may include or exclude vegetable oils, omega-3 fatty acids, omega-6 fatty acids and combinations thereof.
[0061] [0061] Each of the steps mentioned above can present any of the modalities of the step presented in this disclosure, and the method can comprise the processing of additional recyclable chains.
[0062] [0062] In some respects, the recyclable biological chain can be selected from recyclable biological chains, including: bone meal, feather meal, selected vegetables or fruits, grape marc, tomato marc, olive marc, olive marc, fruits, selected grapes, selected tomatoes, selected olives, peanut shells, nut shells, almond shells, pistachio shells, legumes shells, recyclable fresh food and recyclable bakery products. Fresh food recyclables can be provided by obtaining fresh food recyclables collected, for example, from one or more suppliers of fresh or recyclable food waste, for example, supermarkets, butchers, food processing facilities, food distributors fresh, fresh green farm waste, restaurant fat storage containers or other viable sources of recyclable fresh food. In some respects, the provision of fresh food recyclables comprises the collection of recyclable materials from supermarkets, food wholesalers, food processing facilities, institutions (recyclable from food preparation facilities such as sports facilities, schools, hospitals, hotels, snack bars and other institutions) distributors of fresh food, fresh green recyclables from farms or other viable sources of fresh food recyclables. In some respects, fresh food recyclables are provided by the collection of organic products, meat, fish, delicatessen and bakery products, selected by supermarket employees from the food offered for sale in supermarkets. In some respects, the recyclable biological stream can be collected frequently. In some respects, frequent collection intervals can be once, twice or several times a day or once, twice, three times, four times, five times, six times or seven times a week.
[0063] [0063] In some respects, the agricultural mixtures described in this document can be further mixed with organic fertilizers to produce a synergistic effect of organic fertilizers and the agricultural mixtures described in this document to improve crop yield and organic soil content . Organic fertilizers can include or exclude bone meal, blood meal, feather meal, chicken manure and cow manure. The inventors have surprisingly found that the processed agricultural mixtures described in this document, when mixed with an organic fertilizer, provide granulation of the combined product. The inventors have also surprisingly found that the processed agricultural mixtures described in this document, when mixed with an organic fertilizer, result in faster decomposition of the organic fertilizer into nutrients to improve plant and / or crop growth rates and crop yields .
[0064] [0064] Blood meal is the liquid or dried blood of an animal after slaughter. Blood meal has a high nitrogen content, usually up to 15% (by weight), due to its high protein content.
[0065] [0065] In some respects, the agricultural mix produced by the methods described in this document from recyclable streams of soy flour can be combined with an agricultural mix produced from a different recyclable biological stream ("agricultural mix of soy products) ") as described in this document to produce a high protein fertilizer or animal feed. In some respects, the agricultural mixture of the soy-based product comprises a high content of proteins and / or amino acids in the form of amino acids and peptides in the feed. In some respects, the final nitrogen concentration (weight) in an agricultural mixture of soy product is selected from: 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1 , 0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2 , 0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 3.0%, 3.5%, 4.0%, 4.5%, 5 , 0%, 5.5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14%, or any interval between two of the quoted percentages. In some respects, the final nitrogen concentration (weight percent) in the agricultural mixture produced by the methods described in this document can vary from 1-3.0%, 3.0-3.5%, 3.5-4, 0%, 4.0-4.5%, 4.5-
[0066] [0066] In some respects, a recyclable biological stream may include or exclude recyclable products for birds. In some respects, poultry can be selected, for example, from chickens (for example, Gallus gallus domesticus), turkeys (for example, Meleagris gallopavo), quails (for example, callipepla genus), ostrich (for example, struthio camelus ) and emu (e.g., dromaius novaehollandiae). Recyclable poultry products can include or exclude the various components of birds: feathers, beaks, feet, claws, bones and feces. In some ways, an enzyme selected to digest poultry recyclables may include or exclude a protease or keratinase. The agricultural mix produced by the methods described in this document from recyclable biological streams that include recyclable poultry streams ("agricultural poultry mixes") can exhibit a high content of proteins, peptides and / or amino acids, and can be used to improve crop yield or provide digestible protein in animal feed. In some ways, agricultural additives for poultry can be used as fertilizer. In some respects, poultry farm mixes increase the nitrogen content in the soil. In some respects, poultry farm mixes increase crop yields. In some respects, poultry farm mixtures produce a fertilizer product with a high nitrogen content. In some respects, poultry farm mixes are combined with an agricultural mix produced from a different biological recyclable stream, as described in this document to produce a feed with high protein content.
[0067] [0067] In some respects, the selected recyclable vegetables or fruits can be selected from: selected grapes, selected olives, selected corn (for example, zea mays Linn), selected bottle gourd (for example, lagenaria siceraria), selected carrot ( for example, daucus carota), selected peas (for example, Pisum sativum), selected potatoes (for example, Solanum tuberosum L), selected beets (for example, Beta vulgaris var. altissima), selected celery (for example, Apium graveolens) , selected tomato (for example, Lycoper sicon esculentum Mill.), Selected elements of the brassica genus (for example, selected broccoli (for example, Brassica oleracea)), selected radish (for example, Brassica oleracea B), selected cauliflower ( e.g. Brassica oleracea C), selected Brussels sprouts (e.g. Brassica oleracea), selected cabbage (e.g. Brassica oleracea), cabbage (e.g. Brassica oleracea A), sel cabbage ecioonated (for example, Brassica oleracea A), selected mustard (Brassica juncea), selected turnips (for example, Brassica rapa var. Rapa) and selected rutabaga (for example, Brassica napus subsp.
[0068] [0068] In some ways, the recyclable biological stream can be soy or a soy product. Soy can be hydrolyzed by the methods described in this document to produce a soy protein. The term "soy protein", as used herein, means any form of soy concentrate or soy isolate that can be, for example, a commercial soy concentrate or soy isolate or the soy concentrate or soy intermediate produced in a plant adopted for converting defatted soy flour into polypeptides. In some respects, the soy product may include or exclude soy flour. Soy flour is the remaining product after grinding the soy bean, using a physical press to extract the soy oil. In some respects, soy flour comprises 10 to 45% (by weight) of protein. The concentration of soy protein referred to above related to the proteolytic activity of the enzymes described in this document and the concentration of substrate is calculated as the percentage of nitrogen measured according to Kjeldahl multiplied by 6.25. In some respects, the recyclable biological stream may comprise soy and lettuce. The inventors surprisingly found that the hydrolysis of soy requires a source of water and that lettuce provides a plant with a high water content. In some aspects, lettuce hydrolysis is performed using cellulase at an acidic pH in the incubation steps described in this document. In some respects, the hydrolysis of lettuce and / or soy product can be accomplished using a recyclable biological whey stream comprising lactic acid.
[0069] [0069] In some respects, the biological recyclable stream processed by the methods described comprises selected fruits or vegetables ("agricultural fruit / vegetable mixture"). The agricultural fruit / vegetable mix can display selected properties. Mixtures combined with an agricultural fruit / vegetable mix may exhibit selected properties. The selected properties result from the selection of the selected recyclable chain of fruits or vegetables processed by the methods described in this document. In some aspects, the selected fruits may include or exclude: citrus molasses, fruit peel, fruit juice and fruit pulp. In some ways, the vegetables selected may be lettuce. The lettuce content can increase the water content as needed to increase nutrient solubility when the recyclable lettuce stream selected is mixed with other recyclable biological streams. When the other recyclable biological stream comprises recyclable soy products, the water from the lettuce can solubilize the nutrients from the soy residues.
[0070] [0070] In some respects, agricultural mixes prepared from recyclable streams of selected vegetables (eg brassicas (eg broccoli, radish, cauliflower, Brussels sprouts, kale, curly cabbage, green mustard, turnips and / or rutabaga)) are useful as natural pesticides. The inventors have discovered that agricultural mixtures produced by the methods described in this document from recyclable streams comprising brassicas can comprise glycosinolates, which hydrolyze to isothiocyanate. Isothiocyanate is produced from brassicas when the brass cell walls are compromised by the milling and cellulases described in this document. In some aspects, agricultural mixtures comprising isothiocyanate can be concentrated (for example, by filtration, evaporation, lyophilization, nebulization, spray drying) to increase the concentration of isothiocyanate. In some respects, the isothiocyanate concentration can vary from 0.1 to 15,000 mg / kg of the agricultural mixture before concentrating the mixture, or any value in that range, depending on the composition of the recyclable stream. In some respects, agricultural mixtures with natural pesticide properties are applied to plants in part to protect natural pesticides, nematodes and / or weeds. In some ways, agricultural mixtures of natural pesticides can be applied to the leaves, stems or roots of plants. In some respects, the form of isothiocyanate is allyl isothiocyanate.
[0071] [0071] In some respects, agricultural mixtures produced by the methods described in this document may provide a composite product, including separated solids, which are then passed through a screw press (as can be manufactured by Vincent, Doda or Fan); a belt filter (like the one that can be manufactured by Westphalia, Andritz or Westech); or a hydraulic press (as can be manufactured by Pall or Flow Press) to extract liquids, which can be recycled in the biological hydrolyzate, and dehydrated biological particles. The dehydrated biological particles can be used as a source of cellulosic material or fiber in the dry mix of dry animal feed. Dehydrated biological particles can also be used in the production of biofuels. Dehydrated biological particles can also be used to produce green organic compost, or an organic compost made from that material and basalt rock dust.
[0072] [0072] In some respects, the biological recyclable stream selected from fruits and / or vegetables can be used to produce an agricultural mix obtained by the methods described in this document with an increased sugar content compared to an agricultural mix from a recyclable biological stream different. The recyclable biological stream used to produce agricultural mixtures with a high sugar content can be selected fruits or vegetables with a high sugar content (fructose, glucose, xylose, mannose or sucrose). In some ways, the high sugar content that contains fruits or vegetables may include or exclude: apples, pears, cherries, blackberries, oranges, lemons, grapefruits, pomelos, papaya, watermelon, melon, melon, melon, cantaloupe, strawberry, blueberry , raspberry, banana, grapes, blackberries, blackberries, plums, apricots, nectarines, guava, pluotas, pineapples, mangoes and mixtures and combinations thereof. In some respects, the agricultural mixture produced by the methods described in this document with an increased sugar content can be used as an animal feed. In some respects, the agricultural feed mix with high sugar content can be supplied to animals to increase fat production and / or absorption. In some respects, the agricultural feed mix with high sugar content may be mixed or combined with an agricultural mix processed from a different biological recyclable stream at any point in the process of said different recyclable biological stream. The resulting mixture can be used as a feed for animals with a higher sugar content. Animal food with high sugar content can make the agricultural mixture more pleasant to the animal, resulting in a greater amount of food absorption.
[0073] [0073] In some respects, the biological recyclable stream selected from fruits and / or vegetables can be mixed or combined with a recyclable stream of soy product to manufacture an agricultural mix produced by the methods described in this document with an increased nitrogen content. In some ways, increasing the nitrogen-containing agricultural mix can be used as an organic fertilizer.
[0074] [0074] In some respects, agricultural mixtures produced by the methods described in this document can be used to increase the adhesion of pesticides to plant surfaces. In some respects, agricultural mixtures produced by the methods of this disclosure using recyclable biological streams comprising fats can exhibit a high oil content from processed fats. The oils give a liquid agricultural mixture a viscosity. In some respects, the agricultural mixture may be combined with a pesticide prior to application to plants and / or may comprise natural pesticides from mixtures obtained from selected brassicas, as disclosed in the present document. The oils in the agricultural mixture can form a complex between the pesticide and the plant surface to improve the adhesion of the pesticide to the plant or a component of the plant. In some respects, the agricultural mixture with pesticide can be further increased in fat content by adding centrifuged oils separate from the processing of a portion or any other recyclable biological stream. The plant component can be selected from: roots, leaves, stems, fruits, pollen, bark or combinations thereof.
[0075] [0075] In some aspects, pesticides can be adhered to a plant using the agricultural mixtures of this disclosure by a method that comprises the steps of: (a) presenting an agricultural mixture produced by the methods described in this document, where the recyclable stream biological comprises a fat;
[0076] [0076] In some aspects, the plant component can be selected from: roots, stems, leaves, fruits or combinations thereof. Vitamins and Antioxidants in Agricultural Mixtures
[0077] [0077] In some respects, the recyclable biological stream may comprise olive pomace, grapes, tomatoes, cocoa and / or apples. The recyclable bagasse stream can include or exclude seeds, seed peels, fruit peels, peels and residual fruit juice. The recyclable stream of olive, grape, cocoa or tomato pomace can include vitamins and antioxidants.
[0078] [0078] In some respects, vitamins and antioxidants may include or exclude: polyphenol compounds, tocopherol compounds, flavonoids and vitamin C. In some respects, polyphenol compounds may include resveratrol. In some ways, flavonoids are anthocyanins. The inventors have determined that agricultural mixtures produced by the methods described in this document from recyclable bagasse streams ("agricultural bagasse mixtures") exhibit high concentrations of vitamins and antioxidants. In some respects, agricultural mixtures of bagasse can be mixed with agricultural mixtures processed from other recyclable biological streams, as described in this document, to produce animal feed with a higher concentration of antioxidant compounds. The resulting agricultural mixtures with high concentrations of vitamins and antioxidant compounds can be supplied to animals as a method of providing vitamins and antioxidants to animals.
[0079] [0079] In some respects, antioxidants are added to the feed or any step in the process prior to the formation of the feed. Antioxidants are as described in this document.
[0080] [0080] In poultry meat, the bright red or pink color associated with freshness fades to gray-brown, as the oxymyoglobin is converted to methoglobin by oxidation. Lipid oxidation can also occur, affecting the acceptability of aroma and taste. The growth of spoiled bacteria, such as Pseudomonas spp., Exacerbates these effects, in addition to influencing meat texture. Consumers' willingness to buy meat is greatly reduced by these changes. In some respects, the processes described in this document include the addition of an antioxidant to agricultural mixtures that are to be used as animal feed. In some ways, the antioxidant is added using vitamin E as a fat-soluble antioxidant. In some ways, vitamin E is supplied from a bagasse. In some ways, the bagasse that provides vitamin E is tomato bagasse. In some respects, animal feed from the agricultural mixtures described in this document is useful for the production of processed meat comprising antioxidants that decrease meat spoilage and increase the shelf life of processed meat, including broilers. In some respects, the antioxidant supplier of animals, when fed with egg layers, increases the useful life of the egg layer, resulting in an increase in eggs produced by the bird. In some respects, chickens fed the vitamins and animal antioxidant described in this document produce darker colored eggs. In some ways, eggs comprise beta carotene.
[0081] [0081] In some respects, bagasse agricultural mixes provide a source of vitamins and antioxidants in animal feed, by a method comprising: (a) provision of an agricultural mix produced from a recyclable biological stream comprising bagasse in accordance with with the methods described in this document; and b) introduction of the agricultural mixture to animal feed.
[0082] [0082] In some respects, agricultural mixtures of bagasse provide antioxidants to processed meat, by a method comprising: (a) provision of an agricultural mixture produced from a recyclable biological stream comprising bagasse according to the methods described in the present document; (b) introducing the agricultural mixture into an animal feed to feed an animal; c) slaughter of the animal;
[0083] [0083] In some respects, bagasse agricultural mixtures extend the shelf life of processed meat, by a method comprising: (a) provision of an agricultural mix produced from a recyclable biological stream comprising bagasse according to the methods described in this document; (b) introducing the agricultural mixture into an animal feed to feed an animal; c) slaughter of the animal; d) processing the slaughtered animal into processed meat that contains at least 2% fat content; where the vitamins and antioxidants in the agricultural mix are present in the fat of processed meat.
[0084] [0084] The methods of this disclosure for the production of animal feed comprising an agricultural mixture of bagasse are still useful to reduce the cellulose content present in the recyclable bagasse stream, for feeding animals that would otherwise not have the capacity to digest bagasse unprocessed. In some respects, agricultural / animal feed mixes produced from a recyclable biological stream comprising bagasse can be used as chicken feed. In some ways, agricultural additions of high antioxidant in animal feed can extend the shelf life of animal meat products. In some respects, animals fed high-antioxidant agricultural mixtures retain vitamins and antioxidants in the lipids and fatty acids of animal tissues. In some respects, these antioxidants in the tissues of animals that consume high vitamins and feed combined with agricultural antioxidants increase the shelf life of the processed animal's meat. Without being limited by theory, the antioxidant in fats and lipids in processed meat can reduce discoloration, reduce rancidity, decrease spoilage and / or prevent oxidation of animal fats, resulting in a longer shelf life for processed meat. In some respects, this disclosure includes a processed meat product with an extended shelf life comprising antioxidants provided by the feed. In some ways, the processed meat product can be selected from: poultry, pork or fish. Anti-caking agents
[0085] [0085] In some respects, anti-caking agents can be added to dry forms of animal feed or any step in the process prior to the formation of dry forms of animal feed. Anti-caking agents are additives to powder or granular materials to prevent lumps from forming. Anti-caking agents may include or exclude: tricalcium phosphate, powdered cellulose, magnesium stearate, sodium bicarbonate, sodium ferrocyanide, potassium ferrocyanide, calcium ferrocyanide, calcium ferrocyanide, bone phosphate (ie calcium phosphate), sodium silicate, silicon dioxide, calcium silicate, magnesium trisilicate, talc, sodium aluminum silicate, aluminum and potassium silicate, calcium aluminum silicate, bentonite, aluminum silicate, epolidimethylsiloxane stearic acid. Animal food without antibiotics
[0086] [0086] In some respects, agricultural mixtures produced by the methods described in this document can be used as a source of healthy food in a breeding program without antibiotics. In some respects, the breeding program without antibiotics may include weaned pigs and chicks. Animal feed from the agricultural mixtures described in this document (which contain large amounts of fruits and vegetables) can improve the health of weaned young pigs or chicks, compared to conventional corn and soy flour diets.
[0087] [0087] In some respects, agricultural mixtures produced from a first recyclable biological stream can be combined or mixed with a second recyclable biological stream, which can be any other recyclable stream from this disclosure. In some aspects of this disclosure, for example, the first recyclable biological stream may be a recyclable bagasse stream and the second recyclable biological stream may be recyclable from fresh food. In some respects, the mixed agricultural mixture resulting from recyclable bagasse chains and recyclable fresh food chains can comprise antioxidants and be used as digestible animal feed. In some respects, the mixed agricultural mixture resulting from recyclable bagasse streams and a second recyclable biological stream can be used as feed for animals with a high protein content and a high antioxidant content.
[0088] [0088] In some aspects of this disclosure, the recyclable biological stream may be a recyclable stream of carbohydrates. The recyclable chain of carbohydrates can include or exclude bakery recyclables. Recyclable bakery materials can include or exclude baked goods, expired ingredients or expired dough. Recyclable baked products for baking can include or exclude: cakes, pies, donuts, cereals, pasta, breads, sweets, cookies, chips, snacks, pretzels and the like. Expired ingredients can include or exclude: flour, sugar, icing, yeast, cornmeal and burnt or broken products. In some respects, agricultural additives for baking may comprise a high concentration of carbohydrates compared to agricultural additives produced from recyclable streams that are not bakery products. In some respects, carbohydrates in agricultural bread mixes can shorten the drying time to convert a liquid form of the agricultural mix into a solid form of the agricultural mix. Agricultural additives produced using recyclable carbohydrate chains exhibit, for example, a high sugar content and / or improved granulation properties. The enhanced granulation properties can be useful for making a desired form of animal feed. The appropriate form of animal feed can include or exclude: granules, flakes, pastes, cereals and powders. In some respects, agricultural mixtures of carbohydrates can be combined with agricultural mixtures produced from other recyclable biological streams, as described in this document to improve the granulation of the combined agricultural mixture. In some respects, an agricultural mixture described in this document can be combined, mixed, composed, pulverized, ground or dissolved with a recyclable stream of carbohydrate that has not been processed by the enzymatic digestion methods described in this document. The recyclable carbohydrate stream can be dried and then added to a wet or dry form of an agricultural mixture described in this document to produce a dry form of animal feed.
[0089] [0089] In some respects, this disclosure also presents a method for pelletting an agricultural mixture produced from a recyclable biological stream that includes recyclable carbohydrate streams, in granulated fertilizers and animal feed products, by a method that comprises the stages of: a) presentation of the agricultural mixture produced from recyclable chains of carbohydrates in liquid form; (b) introducing the liquid form of the agricultural mixture into a drying apparatus; (c) drying the agricultural mixture; and (d) cutting the dry agricultural mixture into granules.
[0090] [0090] In some respects, this disclosure also features a method of powdering or granulating an agricultural mixture produced from a recyclable biological stream with a carbohydrate source that has not been processed by enzymatic digestion.
[0091] [0091] The agricultural mixtures in this disclosure can be used as feed for animals with a high conversion rate. Animals (pigs and / or chickens that are usually fed a diet of corn and soy flour) can be fed with the liquid or dry agricultural mixtures of this disclosure to gain weight with greater efficiency in the use of food (that is, a greater conversion rate of feed to animal weight). In some respects, animals produce less manure and less diarrhea when fed with the pre-digested composition. Therefore, approximately 100% of the recyclable biological stream processed according to the methods of this disclosure can be used efficiently. In some ways, animals can include or exclude pigs, birds, rabbits, horses, insects, worms and other non-ruminants. In some ways, poultry can include or exclude chicken, turkey, quail, ostrich and emu. In some ways, insects can include or exclude crickets (eg, acheta domesticus) and black soldier flies (eg, hermetia illucens). In some ways, worms can include or exclude earthworms (for example, Oligochaeta), silkworms, moth larvae and mealworms (for example, Tenebrio molitor).
[0092] [0092] In some respects, the systems described in this document may include a heated feed tank. In some ways, the heated feed tank can be configured to be between the incubation tank and the separation tank. In some aspects, the feed tank can be configured to be between the grinding tank and the incubation vessel. In some ways, the feed tank can be configured to be between the incubation vessel and the drying equipment. The feed tank can be lined to allow temperature control. The coated feed tank can be vaporized to increase the rate of temperature increase. In some aspects, the feed tank is heated to a temperature ranging from about 37.7ºC to about 104.4ºC. In some respects, the feed tank is heated to around 71.1ºC.
[0093] [0093] In some aspects, the grinding of recyclable biological chains can be carried out using a rotary knife grinder. In some respects, recyclable biological currents can be further grounded with a low RPM / high torque grinder with grinding action can also be used to further grind the recyclable biological slurry. In some respects, the agricultural mixtures described in this document can be combined with a recyclable bread chain using a knife grinder to produce granulated products.
[0094] [0094] In some respects, the incubated milled biological pulp can be cut with a high shear grinder with shear action, which can comprise, for example, a high shear mixer with a disintegrating head, during all or part of the incubation and pasteurization steps. A high shear mixer disperses or transports a phase or ingredient (liquid, solid or gas) to a main continuous phase (liquid), with which it would normally be immiscible. A rotor or impeller, along with a stationary component known as a stator, or an array of rotors and stators, is used in a tank containing the solution to be mixed or in a tube through which the solution passes, to create shear. The high shear grinder can give the pulp a high shear rate. In some respects, the high shear grinder can be, for example, the ARDE Dicon in-line dispersion grinder or a Silverson Mixer homogenizer. As used in this document, "shear" refers to a cutting action that reduces the size of food particles, increasing their surface area and, therefore, their interaction with enzyme molecules. In some respects, high shear is created by circulating the pulp through a high speed mixer and high shear through all digestion at rates in the range of 105-106 sec-1 or more.
[0095] [0095] This disclosure does not include a garbage disposal as a shear medium.
[0096] [0096] In some respects, the incubation process may include or exclude a magnetic trap. The magnetic trap can pull and / or hold metallic objects that may be present in the recyclable fresh food chain. In some ways, metallic objects can include or exclude coins, twists, buttons,
[0097] [0097] In some respects, the separation step described in this document can be performed using a means to apply differential sedimentation. In some respects, the means of applying differential sedimentation comprise a centrifuge. In some ways, the centrifuge can be a tricanter centrifuge. In some ways, the tricanter centrifuge can be from Flottweg (Germany), US Centrifuge (United States) or Peony (China). The separation stage by centrifugation can control the oil levels of high titration point of the agricultural mixture. Oils with a high titre content can clog the fertilizer feed pipes when administering agricultural additives to crops. It has surprisingly been found that controlling the levels of oils with a high titer content using a centrifugation separation step can improve the administration of the agricultural mixtures described in this document through fertilizer feed pipes for crops. In some respects, the fat content of the agricultural mixture can be controlled using a tricanter centrifuge. In some respects, the fat content of the agricultural mixture can be reduced from 5-12% to 0.2-4% (weight percent) using the centrifugal separation step. In some respects, the fat content can be reduced from 5-12% to about 1-2% (by weight) for the liquid phase using the centrifugal separation step.
[0098] [0098] The tricanter centrifuge can separate the biological hydrolysates described in this document into biological particles and liquid phases comprising one or more phases. In some aspects, the tricanter centrifuge can also separate the liquid phase into an oil-soluble phase and a water-soluble phase. In some respects, the solid particles can be mixed with the dry agricultural mixtures described in this document (or dried and sedimented without mixing) to produce a high protein animal feed suitable for poultry, pigs, fish and pets. The tricanter centrifuge can be adjusted to separate the oils from the water-soluble phase from the solid particles and / or to adjust the oil level (which may include or exclude the total fat content) in the liquid phase. In some ways, oils can be further separated. Additional oil separation can be performed using a system selected from a decanter, distillation apparatus, chromatography and / or oil-water partitioner.
[0099] [0099] In some aspects, the aqueous phase can be concentrated. In some aspects, the dehydrated aqueous phase can be mixed with the separated biological particles in a dry solid. In some aspects, the dehydrated aqueous phase can be mixed with recyclable biological bread streams to produce granulated animal feed suppliers. The concentration of the aqueous phase can be carried out using vacuum evaporation or vibrating filters. Vacuum evaporation removes the solvent from the water and therefore increases the relative concentration of the components of the aqueous phase in relation to the pre-concentration. Vibrating filters can be used to remove water and salts from the aqueous phase. The fertilizers from the agricultural mixtures described in this document require a minimum salt content and, therefore, can be dehydrated using vibrating filters that remove water and saline. Animal feed may require a higher salt content than fertilizer and therefore can be dehydrated using vacuum evaporation, which retains the salt content of the dehydrated product. In some aspects, the aqueous phase can be dehydrated by lyophilization. In some ways, the aqueous phase can be dehydrated using a dehydration drum. In some ways, the dehydration drum is a vacuum dehydration drum. In some respects, the aqueous phase can be dehydrated by azeotropic removal by adding ethanol to form an azeotrope with water, followed by evaporation of the azeotrope, ethanol and water under atmospheric or vacuum conditions. Enzymes and processes for obtaining agricultural additions
[00100] [00100] In some respects, the selected enzymes involved in the incubation step may include or exclude: at least one enzyme to digest proteins, at least one enzyme to digest fats and lipids or at least one enzyme to digest cellulosic material or at least one enzyme to digest other carbohydrates. Selected enzymes may include or exclude: xylanase, asparaginase, cellulase, hemicellulase, glumaiasis, beta-glumaiasis (endo-l, 3 (4) -), urease, protease, lipase, amylase, keratinase, alpha-amylase, phytase, phosphatase aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, alpha-galactosidase, beta-galactosidase, glycoamylase, alpha-glucosidase, kyloxidase, kyloxidase, 3.4. 99), mannosidase, oxidase, glucose oxidase, pectinesteramide enzyme, poglycerase, peroxidase enzyme, polyphenoloxidase, proteolytic enzyme, protease, ribonuclease, thioglycosidase and transglutaminase. These enzymes can be selected, for example, from the group consisting of enzymes originating from microbial fermentation, enzymes derived from animal digestion, enzymes derived from a microorganism and enzymes derived from plants.
[00101] [00101] In some respects, one or more selected enzymes can be added as individual enzymes or combinations of enzymes to the paste at various times and incubated at selected temperatures. In one aspect, one or more selected enzymes is added to the milled biological slurry in a first combination of enzymes comprising at least two of the selected enzymes described in this document and incubated at a first temperature,
[00102] [00102] In one aspect, a first enzymatic combination of the selected enzymes is added during a first incubation step at a first temperature between approximately room temperature (for example, 12.7ºC to about 32.2ºC, including 13.3, 13.8, 14.4, 15, 15.5, 16.1, 16.6, 17.2, 17.7, 18.3, 18.8, 19.4, 20, 20.5, 21, 1, 21.6, 22.2, 22.7, 23.3, 23.8, 24.4, 25, 25.5, 26.1, 26.6, 27.2, 27.7, 28, 3, 28.8, 29.4, 30, 30.5, 31.1, 31.6, 32.2 ° C) at 60 ° C to form an incubation mixture. In some respects, the first temperature is selected from including 13.3, 13.8, 14.4, 15, 15.5, 16.1, 16.6, 17.2, 17.7, 18.3, 18 , 8, 19.4, 20, 20.5, 21.1, 21.6, 22.2, 22.7, 23.3, 23.8, 24.4, 25, 25.5, 26.1 , 26.6, 27.2, 27.7, 28.3, 28.8, 29.4, 30, 30.5, 31.1, 31.6, 32.2, 32.7, 33.3 , 33.8, 34.4, 35, 35.5, 36.1, 36.6, 37.2, 38.3, 38.8, 39.4, 40, 40.5, 41.1, 41 , 6, 42.2, 42.7, 43.3, 43.8, 44.4, 45, 45.5, 46.1, 46.6, 47.20, 47.70, 48.3, 48 , 8, 49.4, 50, 50.5, 51.1, 51.6, 52.2, 52.7, 53.3, 53.8, 54.4, 55, 55.5, 56.1 , 56.6, 57.2, 57.7, 58.3, 58.8 and 59.4ºC or any range between two of the indicated temperatures. In one aspect, the first combination of enzymes can be added at a first room temperature and the enzymatic processes begin as the system is heated to a second temperature.
[00103] [00103] In one aspect, at least a second combination of selected enzymes can be added to the incubation mixture and a second incubation step can be performed at a second temperature between about 35.5 to 62.7ºC. In some respects, the second temperature is selected from: 35.5, 36.1, 36.6, 37.2, 38.3, 38.8, 39.4, 40, 40.5, 41.1, 41 , 6, 42.2, 42.7, 43.3, 43.8, 44.4, 45, 45.5, 46.1, 46.6, 47.20, 47.70, 48.3, 48 , 8, 49.4, 50, 50.5, 51.1, 51.6, 52.2, 52.7, 53.3, 53.8, 54.4, 55, 55.5, 56.1 , 56.6, 57.2, 57.7, 58.3, 58.8, 59.4.60, 60.5 61.1, 61.6, 62.2 or 62.7ºC or any interval between two of the quoted temperatures. The time of the second incubation can be, in some aspects, between about 1 to about 18 hours or more, preferably between 1.2 to 6 hours, more preferably about 1 hour and a half to 2 hours. In some respects, the second incubation time is selected from: 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 , 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3 , 3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4 , 6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5 , 9, 6, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7, 7.1, 7.2 , 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8, 8.1, 8.2, 8.3, 8.4, 8.5 , 8.6, 8.7, 8.8, 8.9, 9, 9.1, 9.2, 9.3, 9.4, 9.5, 9.5, 9.6, 9.7 , 9.8, 9.9, 10, 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9, 11, 11 , 1, 11.2, 11.3, 11.4, 11.5, 11.6, 11.7, 11.8, 11.9, 12, 12.1, 12.2, 12.3, 12 , 4, 12.5, 12.6, 12.7, 12.8, 12.9, 13, 13.1, 13.2,
[00104] [00104] In some respects, the second combination of enzymes may comprise at least one pectinase, at least one protease and alpha-amylase. In some respects, a protease can be added after pectinase and alpha-amylase in a third combination of enzymes. In some respects, the alpha-amylase may be 1,4-alpha-D-gluian glucan hydrolase (e.g., glycogenase).
[00105] [00105] In one aspect, when the recyclable biological stream comprises selected fruits or vegetables, the selected enzymes can be selected from: a cellulase, a pectinase, a ligninase, an amylase and their combinations. In some aspects, pectinase can be selected from: pectolase, pectozyme, polygalacturonase and their combinations. Without being bound by theory, a pectinase breaks down pectin (for example, polymethyl galacturonate) comprising the cell walls of fruits or vegetables. Amylase can be selected from: alpha-amylase, beta-amylase (1,4-aD-glucan maltohydrolase), gamma-amylase (glucan 1,4-a-glycosidase; amyloglycosidase; amyloglycosidase; or exo 1,4-a- glycosidase) and their combinations. Amylase can catalyze the hydrolysis of starch into sugars. Cellulase can break down the cellulose molecule into monosaccharides, such as beta-glucose, or shorter polysaccharides and oligosaccharides. In some aspects, cellulose can be selected from: endocellulases (EC
[00106] [00106] The temperature and pH of an incubation with one or more selected enzymes can be selected in order to optimize or be suitable for the activity of the enzymes in the reaction mixture. In some aspects, a first temperature and pH can be selected to optimize, or be suitable, for the activity of the first or more selected enzymes in a first combination of enzymes, while a second temperature and pH can be selected to allow for optimization or to be suitable for the activity of the selected enzymes in a second selected enzyme combination. In other respects, the timing of a combination of enzymes can be selected to minimize the impact of the enzymes on each other. In one aspect, when a protease is added in combination with another selected enzyme, the protease is added second, so that the protease does not degrade the other selected enzyme.
[00107] [00107] In some respects, after incubating the ground organic pulp with one or more selected enzymes, the incubated ground organic pulp can be heated between about 65 to 82.2ºC, preferably 62-76.6ºC, for about 30 minutes to about 18 hours, preferably about 30 minutes to 2 hours, to further pasteurize the soil's biological paste.
[00108] [00108] Although the constant incubation and shaking and shearing steps are highly likely to reduce the concentrations of pathogens to undetectable levels, a pasteurization step over a temperature and duration range commonly used in pasteurization processes further reduces the risk contamination by pathogens at undetectable levels under the current. detection of pathogens. In some respects, pasteurization is carried out for about 15 minutes to about 1 hour. In some aspects, the pasteurization step is carried out for a time selected between: 15, 20, 25, 30, 35, 40, 45, 50, 55 and 60 minutes. In some respects, the pasteurization step can be carried out in various combinations of temperature, pressure and duration, as commonly used in pasteurization processes. In these aspects, pasteurization can be carried out, for example, from about 15 minutes to about 12 hours, for any period of time in 15 minute intervals between 15 minutes to 12 hours (for example, 15 minutes, 30 minutes, 45 minutes, etc.) or any pasteurization time described in this document. In some respects, the temperature may be about 48.8, 49.4, 50, 50.5, 51.1, 51.6, 52.2, 52.7, 53.3, 53.8, 54 , 4, 55, 55.5, 56.1, 56.6, 57.2, 57.7, 58.3, 58.8, 59.4, 60, 60.5, 61.1, 61.6 , 62.2, 62.7, 63.3, 63.8, 64.4, 65, 65.5, 66.1, 66.6, 67.2, 67.7, 68.3, 68.8 , 69.4, 70, 70.5, 71.1, 71.6, 72.2, 72.7, 73.3, 73.8, 75, 75.5, 76.1, 76.6, 77 , 2, 77.7, 78.3, 78.8, 79.4, 80, 80.5, 81.1, 81.6, 82.2ºC or more, or any temperature or interval that falls between two of these temperatures . In some aspects, pasteurization can be carried out with a pressure of 1 to 10 atm (atmospheres). In some respects, pasteurization can be carried out at a pressure of 1, 2, 3, 4, 5, 6, 6, 7, 9 or 10 atm.
[00109] [00109] The separated incubated biological hydrolyzate may contain small incubated biological particles. In some respects, the separation of step (d) produces a liquid hydrolyzate that is about 90% to about 95% by weight relative to the weight of the inlet that incubates biological recyclable materials and particles with an average diameter of less than 1,000, 950, 900, 850, 841, 800, 750, 707, 700, 650, 600, 590-595, 550, 500, 450, 400, 354, 350, 300 or 271 microns (or any interval between any of the aforementioned diameters ). In some respects, the particles have an average diameter greater than 250, 210, 200, 177, 175, 150, 149, 125, 105, 100, 90, 88, 85, 75, 74, 63, 60, 53, 50, 44, 40 or 37 microns (or any interval between any of the aforementioned diameters). The average particle diameter can be measured using light scattering (for example, scattering laser light at various angles). In some respects, the average particle diameter is measured using a Wyatt Technologies Dawn Heleos II instrument (Wyatt Technologies, Inc., Santa Barbara, CA, USA).
[00110] [00110] The separated incubated biological hydrolyzate can be emulsified using an ultra high shear grinder. Emulsification can produce a homogeneous solution so that the viscosity of any three samples is measured to be within an experimental error of each other. The ultra-high shear grinder can be designed for maximum shear and low current. In some respects, the ultra-high shear grinder can be, for example, a suitable grinder for polishing what has been captured. In some respects, the ultra-high shear grinder can be, for example, an ultra-high shear multistage mixer with maximum shear and low current. In one aspect, the emulsified hydrolyzate produced using an ultra-high shear mixer has an average particle size of less than 70, 65, 60, 55, 50, 45, 40, 35, 30, 29, 28, 27, 26 or about 25 microns or less, or 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11 or 10 microns or less, or any interval between two sizes mentioned, preferably about 26 microns or less, or any emulsion created mechanically or created through the use of emulsifying agents. The particle size can be measured, for example, with the scattering of laser light, as described in this document.
[00111] [00111] In some ways, the processes of this disclosure inactivate pathogens in the recyclable biological stream or in the environment. The methods of this disclosure are therefore useful in the elimination of pathogens present in recyclable biological streams during the production of compositions that can be used safely as fertilizers for the production of products, other crops, fruits, nuts, flowers and grass or as food for animals.
[00112] [00112] In some respects, the grinder used to grind recyclable biological chains can be a rotary knife grinder, which produces particles in the biological particle paste with an average size of about 1/2 inch. In some respects, the ground organic pulp is pumped to a low RPM / high torque grinder in line with grinding action to further ensure that the ground organic pulp has an average particle size of about 1/2 inch or less. The low RPM / high torque grinder can be used in any process for any system with low throughput levels, but is particularly suitable for use in a high throughput processing system, for example, a system capable of processing over 50 tonnes per day, for example, more than 90 tons / day or up to 35 or 100 tons per day or more. The ground biological paste produced by the first grinder, or the first grinder and the optional second grinder, is then pumped into a controlled temperature incubation vessel, where it undergoes constant mixing and incubation with combination (s) of enzymes at the desired temperatures.
[00113] [00113] In addition, the incubation container may contain a recirculation line connected to an in-line grinder with shear action that is used during all or part of the incubation and pasteurization. This may be the third grinder in development aspects, where an optional in-line grinder with grinding action is used to further grind the ground organic pulp, but it is the second grinder in development aspects where the optional grinder is not used. In some respects, the in-line grinder used during all or part of the incubation with the combination of enzymes comprises a high shear mixer. In some respects, the inline grinder comprises a high shear mixer with a disintegrating head. In some respects, the high shear line grinder is used from about 30 minutes to 1 hour after the start of incubation and continues in the pasteurization step. In some respects, start and run times may vary and still achieve the same particle size reduction goals. In some respects, the particles in the resulting incubated biological hydrolyzate can be less than 0.16 cm and about 0.079 cm (1/16 and about 1/32 inch). In some respects, the particles in the resulting incubated biological hydrolyzate can be around 0.24 cm, 0.32 cm, 0.95 cm (3/32, 1.8 or 3/8 inches).
[00114] [00114] The processes described in this document can produce biological particles. In some embodiments, biological particles comprise bone, cellulose, solidified or semi-solid fats, nutshells, fish scales, teeth, inorganic minerals, species containing keratin or combinations thereof. In some respects, species containing keratin are selected from: beaks, feathers, claws or hair. Without being bound by theory, solidified fats can result from incomplete hydrolysis of fat, or fats that are soluble at the incubation temperature, but become solid or semi-solid after cooling. In some embodiments, the levels of biological particles (for example, solid or semi-solid fats) can be controlled using controlled centrifugation processes. In some embodiments, controlled spin processes may include or exclude a fixed number of spin speeds, one or more steps, a ramp spin speed between two or more different spin speeds and one or more spin times.
[00115] [00115] The incubated biological particles that are filtered through the coarse sieve, with an average diameter greater than about 590 microns, may be suitable for use as animal feed and / or as a food supplement or other nutrient source for carnivorous or omnivorous mammals such as pigs, chickens or pets. Compositions of incubated biological particles are digestible and have a high rate of conversion of food to animal weight and / or high nutritional value for pets. The incubated biological particles filtered through the fine sieve can be added to the next batch with a selected recyclable biological stream for further processing. In some respects, the agricultural mixtures in this disclosure can be used as feed for animals with a high rate of conversion of feed to animal weight.
[00116] [00116] In one aspect, the methods described in this document may include a stabilization step after incubation of the ground biological paste with one or more selected enzymes. In one aspect, the stabilization step can occur after the separation of the biological particles from the biological hydrolyzate. In some respects, the dehydrated aqueous phase separated and produced from the centrifugal separation step can be stabilized before dehydration or after dehydration by adding a stabilizer. In some aspects, the stabilization step may also include a preservation step, for example, using inorganic acid, organic acid, inorganic preservatives or organic preservatives, emulsifiers or dispersants, including those that are permitted for use in the production of a certified organic product hydrolyzed. In some respects, the separated particles can be stabilized by drying them. Drying can be carried out by exposing the particles to heat, air, vacuum, vibrating filters or a combination of them. In some aspects, the separated particles can be stabilized when the particles are moistened with a mixture of a stabilizer or preservative, as described in this document. In some respects, the process stabilization step of this disclosure comprises adding and mixing the liquid hydrolyzate with an acid source consisting of hydrochloric, sulfuric, phosphoric, acetic, stearic, propionic, tartaric, maleic, benzoic, succinic, lactic acid or citric acid, preferably phosphoric acid. Lactic acid, acetic acid, citric acid or other certified organic acids can also be used preferably to produce certified organic fertilizers. For example, phosphoric acid or lactic acid can be added to lower the pH of the composition to inhibit microbial and / or pathogenic activity during storage and transportation of the composition that protects nutrients from digestion and / or further degradation by microbes or pathogens. In some ways, phosphoric acid may be tricalcium phosphate. In some respects, the pH of the stabilized liquid hydrolyzate is less than about 3.5. In some respects, the pH of the stabilized liquid hydrolyzate agricultural mixture is about 2.5 to about 3.5, preferably about 3.0. In some respects, the pH of the agricultural liquid hydrolyzate mixture is selected from: 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3, 2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9 or 4.0 or between any of the pH levels mentioned above. The stabilized product can be quarantined overnight, while the content is tested to ensure the elimination of pathogens. Without being bound by theory, the stabilization step can produce a finished product that is stable on the shelf for at least two years, which can be accomplished through any of a variety of stabilization steps, as described in this document.
[00117] [00117] Although the pasteurization step deactivates any bacteria or other pathogen present in the recyclable biological stream or in the processing plant, stabilization prevents the growth of pathogens from environmental sources after the pasteurization step. Without stabilization, microbes and pathogens can contaminate and degrade a liquid hydrolyzate, even after sterilizing the hydrolyzate. The stabilized product is buffered in the soil at a pH similar to the pH of the soil, which, under normal circumstances, will cause the pasteurized liquid hydrolyzate to become biologically active, which is the desired mode of action for the product.
[00118] [00118] A preservative ("stabilizer") such as sorbic acid, potassium sorbate, tocopherol, d-alpha-tocopherol acetate, resveratrol, rosemary oil, erythorbic acid, sodium erythorbate, sodium ascorbate, iso-ascorbic acid, sodium iso-ascorbate, potassium nitrate, ethyl lauroyl arginate, benzoic acid, ascorbyl palmitate, ascorbyl stearate, sulfuric acid, methyl-p-hydroxy benzoate, methyl paraben, potassium bisulfite, potassium lactate, lactate sodium, sodium diacetate, butylated hydroxyanisole (a mixture of 2-butyl tertiary-4) -hydroxyanisol and 3-butyl tertiary-4-hydroxyanisole), butylated hydroxytoluene (3,5-butyl dithercary-4-hydroxytoluene), potassium metabisulfite , propyl-p-hydroxybenzoate, calcium propionate, calcium sorbate, mono and diglyceride esters, dimethyl dicarbonate, natamycin, propyl gallate, potassium sulfate, thyme extract, potassium benzoate or any other suitable food additive preservative can also optionally be added as a preservative during the stabilization step. For organic fertilizers, tocopherol, D-alpha-tocopherol acetate, natamycin, extracted potassium sulfate or any other food preservative certified for organic use can be added as a preservative. In some respects, the preservative is in a concentration of 0.1 to about 2.0%, preferably 0.25% (weight percent) in the agricultural mixture. In some aspects, the preservative concentration is 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1 , 1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0 (percentage by weight) or any percentage by weight between any one of the weight percentages mentioned above. In some ways, tocopherol or D-alpha-tocopherol acetate is added at levels ranging from 10 to 500 mg / kg, or any amount between these values. Natamycin can be added, in some embodiments, at levels, for example, from 0.1 to 100 mg / mL, or any amount between those values. In another embodiment, other preservatives can be added (along with the preservatives listed above, "preservatives") and / or preservatives approved for use in certified organic products ("organic preservatives"). In another aspect, this disclosure refers to agricultural mixtures made of recyclable biological materials, comprising nutrients released by grinding, shearing, homogenization and enzymatic digestion and an acid stabilizer, in which the emulsified hydrolyzate has an average particle size of less than about 26 microns and a pH between about 2.5 and about 3.5, preferably about 3.0.
[00119] [00119] In one aspect, this disclosure refers to methods for collecting recyclable biological currents in a manner of manipulation and timing that does not allow the recyclable biological current to become putrescent.
[00120] [00120] In some respects, the incubated biological particles produced in step (e) in the methods described in this document can be used as animal feed or as a nutrient supplement. In some respects, the agricultural mixtures described in this document comprise an energy content for animal feed. The dry matter of dry liquid agricultural mixtures can vary from 16 to 30% by weight. The crude protein in dry liquid agricultural mixtures can vary from 18 to 40% by weight. The gross energy of dry liquid agricultural mixtures can vary from 5,000 to 8,000 kcal / kg. The percentage of ash from dry liquid agricultural mixtures can vary from 3 to 10% by weight. The acid-hydrolyzed ether extract of dry liquid agricultural mixtures can vary from 1 to 9% by weight. The nitrogen-free extract of dry liquid agricultural mixtures can vary from 5 to 60% by weight.
[00121] [00121] In some respects, the incubated biological hydrolyzate can be emulsified using an ultra-high shear mixer to produce an emulsified biological hydrolyzate. In some aspects, an emulsifying agent can be added to the incubated biological hydrolyzate to form an emulsion.
[00122] [00122] In one aspect, the agricultural mixtures in this disclosure are suitable for use as a fertilizer and for soil correction. The high concentration of nutrients in the agricultural mix provides nutrients directly to plants (including amino acids) and also increases organic matter in the soil, providing nutrients to soil organisms. Those soil organisms that obtain nutrients from the agricultural mixtures in this disclosure grow and promote plant growth, by fixing nitrogen or providing additional organic nutrients to the plants and improving soil quality. For example, liquid hydrolysates comprising amino acids, fatty acids, sugars and minerals not only provide nutrients directly to plants, but also improve the soil, supporting soil organisms, including earthworms and microorganisms, including, for example, nitrogen-fixing organisms ( eg bacteria and archaea) and aerobic bacteria and fungi (eg mycorrhizae), nematodes, protozoa and a variety of invertebrates. The amount of soil organisms increases after application of the biological hydrolysates described in this document. The amount of soil organisms can be measured using carbon dioxide respiration, using the methods described in Kallenback and others (Nature Comm., Published online 28 November 2016, doi:
[00123] [00123] In some respects, the agricultural mixtures described in this document comprise nutrients. Nutrients can include or exclude amino acids (indispensable and expendable amino acids), macro minerals, micro minerals, carbohydrates, saturated fatty acids and unsaturated fatty acids. Amino acids can include or exclude arginine, histidine, isoleucine, leucine, lysine, methionine, threonine, phenylalanine, tryptophan, valine, alanine, aspartic acid, cysteine, glutamic acid, glycine, proline, serine and tryptophan. In some respects, the range of arginine in the agricultural mixture can be from 0.5 to 5% by weight, preferably 1.0 to 1.5% by weight; the range of histidine can be from 0.2 to 5% by weight, preferably 0.5 to 1.0% by weight; the range of isoleucine can be from 0.2 to 5% by weight, preferably 0.5 to 1.5% by weight; the leucine range can be from 0.5 to 10% by weight, preferably 1.3 to 2.0% by weight; the lysine range can be from 0.2 to 5% by weight, preferably 1.0 to 2.0% by weight; the methionine range can be from 0.2 to 5% by weight, preferably 0.4 to 1.0% by weight; the threonine range can be from 0.2 to 5% by weight, preferably 0.7 to 1.5% by weight; the phenylalanine range can be from 0.2 to 5% by weight, preferably 0.5 to 1.5% by weight; the tryptophan range can be from 0.03 to 5% by weight, preferably 0.1 to 3.0% by weight; the range of valine can be from 0.1 to 5% by weight, preferably 0.7 to 1.5% by weight; the range of alanine can vary from 0.1 to 5% by weight, preferably 0.7 to 1.8% by weight; the range of aspartic acid can be from 0.2 to 5% by weight, preferably 1.5 to 2.5% by weight; the cysteine range can be from 0.03 to 5% by weight, preferably 0.1 to 0.3% by weight; the range of glutamic acid can be from 0.2 to 10% by weight, preferably 2.5 to 4.0% by weight; the glycine range can be from 0.2 to 10% by weight, preferably 1.0 to 2.0% by weight; the proline range can be 0.01 to 5% by weight, preferably 0.03 to 1.5% by weight; the range of serine can be from 0.1 to 5% by weight, preferably 0.5 to 1.0% by weight; and / or the tryptophan range can be 0.1 to 5% by weight, preferably 0.4 to 1.0% by weight.
[00124] [00124] When the soil organisms that obtain nutrients from the agricultural mixtures of this disclosure die, they decay and, in turn, provide more organic nutrients to the soil organisms and plants, providing organic matter and additional nutrients to the plants during a sustained period of time and increasing soil organic matter Increasing soil organic matter stimulates the growth, flowering and fruiting of plant roots and increases crop yields. In one respect, the agricultural mixtures in this development can more than double the organic matter in the soil. In some respects, the agricultural mixtures in this disclosure can increase the organic matter of the soil by up to 150%) or more, preferably increasing the organic matter of the soil between about 10% and about 150%, depending on the initial level of organic matter of the ground.
[00125] [00125] Agricultural mixtures produced from selected recyclable biological streams contain more nutrients than compost mixtures obtained using standard composting processes that take up to 3 months and result in the degradation of organic nutrients, resulting in reduced carbon content by conversion to carbon dioxide (CO2), methane (CH4), ethanol (C2H5OH), hydrogen sulfate (H2S) and other by-products related to the decomposition and fermentation effluents.
[00126] [00126] In some respects, the methods described in this document are performed under aerobic conditions, with little decomposition. In some respects, the methods described in this document are performed in the presence of added oxygen during the incubation and / or pasteurization steps. Oxygen can be added by spraying the incubation solution with oxygen gas. Oxygen can be introduced in an amount between about 0.1 and 10 atm. In some respects, the amount of oxygen added is selected from: 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 , 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3 , 2,4, 2,5, 2,6, 2,7, 2,8, 2,9, 3, 3,1, 3,2, 3,3, 3,4, 3,5, 3,6 , 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9 , 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6, 6.1, 6.2, 6 , 3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7, 7.1, 7.2, 7.3, 7.4, 7.5, 7 , 6, 7.7, 7.8, 7.9, 8, 8.1, 8.2, 8.3, 8.4, 8.5,
[00127] [00127] In some respects, the methods described in this document are carried out, for example, in less than 2 to about 12 hours or more, for example, about 3 to about 4 hours, preferably about 3 hours. Use of agricultural mixtures to improve harvest and / or produce yields
[00128] [00128] In another aspect, the application of the agricultural mixtures of this disclosure as a hydrolyzate-based fertilizer can eliminate or reduce the use of conventional nitrate or ammonia fertilizers, such as urea nitrate, ammonium nitrate, calcium and ammonium nitrate or other nitrate or ammonia-based fertilizers, in addition to improving crop yields compared to the use of nitrogen fertilizers only. The agricultural mixtures in this disclosure can promote faster initial growth after germination, increase root growth, increase canopy growth, increase field and / or greenhouse crop yields and / or increase product quality or flavor in relation to the use of nitrogen fertilizers alone, for example, by increasing levels of sugar and / or other flavoring components. In addition, when the fertilizers of this disclosure are used in combination with nitrate or ammonia-based fertilizers, plant growth is improved, including, for example, more vigorous root growth to form more extensive root systems. This results in the absorption of a higher percentage of nitrate or ammonia-based fertilizers by more extensive root systems of the treated plants, further reducing the amount of drained nitrate in addition to the reduction in the amount of applied nitrate or ammonia fertilizer and increasing the water and nitrate use efficiency. In addition to polluting groundwater and causing eutrophication of aquatic water in the country's waterways, the excessive use of nitrate or ammonia-based fertilizers also causes the release of nitrous oxide (N2O), a greenhouse gas that is more than 300 times more harmful than carbon dioxide, according to the United States Environmental Protection Agency (EPA).
[00129] [00129] In one aspect, the fertilizers in this disclosure can be applied using drip irrigation pipes. In some respects, the agricultural mix stock fertilizers in this disclosure are diluted before use. For example, agricultural mixtures can be diluted with water to 1/5, 1/6, 1/7, 1/8, 1/9, 1/10 or, in some applications, to only 5%, 4%, 3 %, 2% or 1% or less before use. In some respects, agricultural mixtures can be presented as dry powder and dissolved in water before use. Preferably, the agricultural mixture is diluted to 1/10 or as low as 1% (by weight) or less before use. In some respects, the suitability of the agricultural mixtures in this disclosure for use with drip irrigation without obstructing the drip lines results from grinding and emulsifying particles soluble in water and oil in the hydrolysates. Washing and / or cleaning the drip lines with water below may also be desirable to prevent the growth of microbes in the drip lines after applying the hydrolysates of this disclosure. In some aspects, the agricultural mixture is applied to crops by spraying, preferably through a sprinkler. In some respects, the agricultural mixture is mixed with a soil splice, for example, fertilizer or rendering by-products, before the application of the ground splice ground before or during the growth of the harvest.
[00130] [00130] In another aspect, this disclosure refers to a method for increasing the yield of the product, the method comprising applying drip line irrigation to a composition comprising agricultural additives made from selected recyclable biological streams, the agricultural additive comprising nutrients released by milling, shearing, homogenization and enzymatic digestion and an acid stabilizer, in which the agricultural mixture has an average particle size of less than about 30 microns and a pH between about 2.5 and 3.5, where the yield of the product is increased by at least 10% in some crops, and more than 40% in other crops, compared to treatment with nitrate or ammonia-based fertilizers only. In some respects, the agricultural mixture (diluted) is applied in combination with nitrate or ammonia-based fertilizers, either by separate application in the same or different schemes, or by combining the mixture and nitrate or ammonia-based fertilizer in a mixture. For example, the agricultural mixture can be applied in a mixture of 90: 10, 85: 15, 80:20, 75:25, 70:30, 65:35, 60:40, 55:45 50:50, 45: 55, 40:60, 35:65, 30:70, 25:75, 20:80, 15:85 or 10:90 (v / v) or any ratio between any of the reasons mentioned above or in that proportion in combination with a fertilizer based on nitrate or ammonia.
[00131] [00131] In some respects, the application of the fertilizers in this disclosure increases the yield of the crop compared to the use of nitrate fertilizers alone, as described in this document, even when the amount of nitrate or ammonia based fertilizer is reduced. Preferably, the use of the hydrolysate-based fertilizers in this disclosure increases the yield of the crop compared to nitrate fertilizer only by at least 10%, 15%, 20%, 25%, 30%, 35% 40%, 45%, 50%, 60%, 70%, 80%, 90%, 100%, 200%, 300%, 400% or at least 10% during a growing season.
[00132] [00132] In some respects, agricultural mixtures produced by the methods described in this document can be combined or mixed with a dispersant to prevent fats and / or oils in the mixture from being adsorbed on the release pipes, thereby improving the current through the release pipes when the agricultural mixture is applied as a fertilizer for crops. It has been surprisingly found that the addition of a dispersant to the agricultural mixture also significantly improves the formation of emulsions in the agricultural mixture. It was found that emulsion formation in low fat agricultural mix liquids was difficult without the addition of a dispersant, because the fat provided the source of hydrolyzable lipids, which was the main source of surfactant in a medium without dispersant. .
[00133] [00133] In some respects, the dispersant can be a surface active agent. The surfactant may include or exclude: polyethylene glycol alkyl ethers, octaethylene glycol monododecyl ether, pentaethylene glycol monododecyl ether, glycoside alkyl ethers, decyl glycoside, lauryl glycoside, octyl glycoside, polyethylene glycol, ether glycol, ether , Nonoxynol-9, glycerol alkyl esters, glyceryl laurate, polyoxyethylene glycol sorbitan alkyl esters, sorbitan alkyl esters, Cocamide MEA, dodecyldimethylamine oxide, cetrimonium bromide (CTAB), cetylpyridate (CPC) chloride BAC), benzethonium chloride (BZT), dimethyldioctadecylammonium chloride, dioctadecyldimethylammonium bromide (DODAB), docusate (sodium dioctyl sulfosuccinate), perfluoroctane sulfonate (PFOS), perfluorobutanesulfate, alkylsulfate, is alkyl aryl sodium lauroyl sarcosinate, Perfluoroctanoate (PFOA or PFO), lauryl sulfat ammonium, sodium lauryl sulfate, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, Arkopal N-300 (C9H19C6H4O (CH2CH2O) 30H), Brij 30 (linear chain polyoxyethylene alcohol), Brij 35 (C12H25O (CH2CH2O) C16H33O (CH2CH2O) 10H), Brij 58 (C16H33O (CH2CH2O) 20H), EGE Coco (ethyl glycoside), Genapol X-150 (C13H27O (CH2CH2O) 15H), Tergitol NP-10 (nonylphenoxide), Marlipal 013/90 ( (CH2CH2O) 9H), Pluronic PE6400 (), Sapogenat T-300 (C4H9) 3C6H2O (CH2CH2O) 30H), T-Maz 60K (ethoxylated sorbitan monostearate), T-Maz 20 (ethoxylated sorbitan monolaurate), Triton X- 45 (C8H17C6H4O (CH2CH2O) 5H), Triton X-100 (C8H17C6H4 (OC2H4) 10OH), Triton X-102 (C8H17C6H4O (CH2CH2O) 12H), Triton X-114 (C8H17C6H4O (CH2CH2O) XH-7.5H) 7.5H) (C8H17C6H4O (CH2CH2O) 16H), Tween 80 (C18H37-C6H9O5- (OC2H4) 20OH), Cocamidopropyl betaine, ethoxylated nonylphenol, Diethanolamine, propylene glycol, oleic acid sorbitan monoester, coconut oil monoethanolamine , a polyethoxylated tallow mine, dipropylene glycol methyl ether and combinations thereof.
[00134] [00134] In some aspects, the dispersant can be added to the incubated mixture during incubation to solubilize fats and oils. In some aspects, the dispersant can be added before or during the emulsification step to produce an emulsified agricultural mixture comprising the dispersant.
[00135] [00135] In some respects, agricultural mixtures produced by the methods described in this document can be processed according to the methods disclosed in this document, combined or mixed with an inorganic mineral to create a mixture with a high mineral content. In some ways, the inorganic mineral is combined with the processed agricultural mixture. The resulting mixture with inorganic mineral can feed microbes with organic and inorganic nutrients to synergistically improve nutrient uptake directly and indirectly (from microbes) in the plant's rootstock.
[00136] [00136] In some respects, the agricultural mixtures described in this document can be effective in increasing crop yields compared to nitrate fertilizers under highly stressful growing conditions. In some respects, highly stressed growing conditions may comprise high salinity soil. In some respects, highly stressed growing conditions may comprise reduced amounts of water irrigation. In some ways, highly stressed growing conditions can include irrigation with high salinity water. In some respects, highly stressed growing conditions may comprise low soil water content (including growth during or after a drought), high temperature (over 32.2ºC) and / or soils with low levels of micronutrients. The agricultural mixtures described in this document increase the population of microbes in the soil, which in turn increases the uptake of saline from irrigation water and / or soil, which reduces the exposure of saline to crops. The agricultural mixtures described in this document can be used to increase the area of usable arable land.
[00137] [00137] In some respects, the application of fertilizers from this disclosure increases crop yields compared to nitrate fertilizers under conditions of high salinity. The soil can comprise a high mineral content that is otherwise inhospitable for plant growth. Fertilizer application can promote harvest yield when applied to the soil before or during plant growth. The inventors have determined that agricultural mixtures produced by the methods described in this document can be used to protect crops from irrigation with high salinity water. In some respects, crops fertilized with agricultural additives produced by the methods described in this document can be irrigated with high salinity water, comprising up to 200 ppm (parts per million) of sodium chloride (NaCl) without reducing crop yields compared to crops irrigated with normal water and a control nitrate fertilizer (for example, obtaining "high crop yields" compared to the yields that would be obtained when irrigating with high salinity water when using common fertilizers).
[00138] [00138] In some respects, higher crop yields than nitrate fertilizers can be obtained on high salinity soil using the agricultural mixtures in this disclosure by a method that comprises the steps of: (a) providing an agricultural mix produced by methods described in this document; (b) applying the agricultural mixture to a plant; and (c) irrigation of the plant with water.
[00139] [00139] In some ways, beneficial soil microbes or fungi can be added to the agricultural mix before applying the mix to crops. This can be done in a fermentation tank in the water, where temperature, pH, oxygen levels and agitation are maintained at levels to maximize the increase in microbial colony count before the production of that fermentation tank is sent to the farms for application to crops through its irrigation system. Beneficial soil microbes, beneficial bacteria and / or beneficial fungi may undergo colony expansion between processing time and presentation to crops. Alternatively, the agricultural mixture can be applied to the soil using agricultural irrigation equipment, followed shortly after by the application of microbes or fungi beneficial to the soil, thus facilitating the increase in the expansion of colonies for these microbes. In some aspects, when soil microbes are added to the agricultural mixture, the stabilization step may not be carried out in order to guarantee the viability of the microbes. BRIEF DESCRIPTION OF THE FIGURES
[00140] [00140] Figure 1 is a flowchart that shows the process steps involved in a modality of this disclosure. A first recyclable biological stream is subjected to a grinding and shearing step to produce a first biological paste. The first biological paste is incubated with one or more selected enzymes. An optional second recyclable biological stream is subjected to a grinding and shearing step to produce an optional second biological pulp. The optional first and second biological pastes are then mixed to form a mixed first and second biological pastes. The mixture of the first and second biological pulp is then incubated with one or more selected enzymes and then pasteurized to produce a mixed biological pulp comprising mixed biological hydrolyzate and mixed biological particles. The first biological paste is then subjected to a separation step, producing the first biological particles and the first biological hydrolyzate. The first biological hydrolyzate is then subjected to a stabilization step that produces a first stabilized biological hydrolyzate. The first stabilized biological hydrolyzate is then emulsified to form a first agricultural mixture. The first agricultural mixture is optionally subjected to a drying step to produce a first dry agricultural mixture.
[00141] [00141] Figure 2 is a flow chart that shows the process steps involved in a modality of this disclosure from a first recyclable chain as an example. Additional recyclable chains can be incorporated at any point in the process steps, with a corresponding process output. The first biological paste is then subjected to a separation step, producing the first biological particles and the first biological hydrolyzate. The first biological hydrolyzate is then subjected to a stabilization step that produces a first stabilized biological hydrolyzate. The first stabilized biological hydrolyzate is then emulsified to form a first agricultural mixture. The first agricultural mixture is optionally subjected to a drying step to produce a first dry agricultural mixture.
[00142] [00142] Figure 3 is a flowchart showing the process steps involved in one embodiment of this disclosure from selected first and second recyclable biological streams, in which the mixed biological hydrolyzate is stabilized to form the stabilized mixed biological hydrolyzate when it is then emulsified and dried to obtain a dry form of the agricultural mixture.
[00143] [00143] Figure 4 is a flowchart that shows the process steps involved in a modality of this revelation from selected first and second recyclable biological streams, in which agricultural mixtures are mixed together near the end of the process. A first recyclable biological stream is processed according to the steps described in figure 1 to produce a first agricultural mixture. A second recyclable biological stream is processed according to the steps described in figure 1 to produce a second agricultural mixture. The first a and second agricultural mixtures are then mixed to form a mixed agricultural mixture. The mixed agricultural mixture is then optionally subjected to a drying step to produce a mixed dry agricultural mixture.
[00144] [00144] Figure 5 is a flowchart showing the process steps involved in a modality of this disclosure from the first and second recyclable biological streams selected, in which the stabilization step is carried out after the pasteurization step and before the separation.
[00145] [00145] Figure 6 is a flowchart showing the process steps involved in a modality of this disclosure from the first and second recyclable biological streams selected, in which the stabilization step is carried out in the first and second biological hydrolysates after the separation and before the emulsification step.
[00146] [00146] Figure 7 is a flowchart showing the process steps involved in a modality of this disclosure from the first recyclable biological streams selected, where the first biological particles are combined with the dry agricultural mixture and bread products optionally separated to produce Animal Food (V).
[00147] [00147] Figure 8 is a flowchart showing the process steps involved in a modality of this revelation from selected first and second recyclable biological streams, in which the first biological particles are combined with the dry agricultural mixture and mixture of the first and second second recyclable biological streams to produce Food for Animals (VI).
[00148] [00148] Figure 9 is a flow chart showing the optional steps of separating the biological hydrolyzate into one or more liquid phases and solid particles. The one or more liquid phases can optionally be separated into an oil phase and an aqueous phase. The oil phase can optionally be mixed in the stabilized biological hydrolyzate. The aqueous phase can optionally be mixed in the stabilized biological hydrolyzate. The separated biological particles can optionally be mixed in the stabilized biological hydrolyzate.
[00149] [00149] Figure 10A is a graph showing that the agricultural mix compositions of this invention protect plants against stress from high salt content. The yield of the strawberry harvest as a function of the harvest time for four different harvest sections, where each section is treated separately with water, 200 ppm brine water, Grower's Standard control fertilizer, Grower's Standard with an agricultural mixture of this applied revelation in an amount of 5 gallons per acre, and Grower's Standard with an agricultural mixture of this development applied in an amount of 10 gallons per acre.
[00150] [00150] Figure 10B is a graph that shows the accumulated revenue per acre of cohorts treated with agricultural mix compositions of this invention in relation to Grower's Standard.
[00151] [00151] Figure 11A shows graphs of the average concentration of nitrate in the leachate from bioassay chambers treated with organic bone meal ("bone") fertilizers in combination with H2H. Referring to the points on day 28, the graphs for bone, H2H, bone + H2H and water are shown in descending order.
[00152] [00152] Figure 11B shows graphs of the average concentration of nitrate in the leachate from bioassay chambers treated with organic feather meal fertilizers ("feathers") ("bones") in combination with H2H. Referring to the points on the 28th, the graphs for feather + H2H, feather, H2H and water are shown in descending order.
[00153] [00153] Figure 11C shows graphs of the average nitrate concentration in the leachate from bioassay chambers treated with organic blood meal ("blood") fertilizers in combination with organic fertilizers. Referring to the points on the 28th, the graphs for H2H, blood, water and blood + H2H are shown in descending order.
[00154] [00154] Figure 12A shows bar graphs of the average nitrate concentrations (in ppm) in the leachate after day 3 in the bioassay chambers of experiment # 1 treated only with organic bone meal fertilizer and in combination with H2H. Bars are standard error of measurement and asterisks indicate statistical differences P <0.05.
[00155] [00155] Figure 12B shows bar graphs of the average nitrate concentrations (in ppm) in the leachate after day 14 (figure 12B) in the bioassay chambers of experiment # 1 treated only with bone organic fertilizer and in combination with H2H. Bars are standard error of measurement and asterisks indicate statistical differences P <0.05.
[00156] [00156] Figure 13 shows a graph of the average ammonium concentrations in the amendments of the bioassay chambers of experiment # 1 treated only with organic bone meal fertilizer and in combination with H2H. Referring to the points on the 28th, the graphs for water, bone + H2H, bone and H2H are shown in descending order.
[00157] [00157] Figure 14 shows a bar graph of the average ammonium concentrations in the leachate from the bioassay chambers of experiment # 1 treated only with organic feather meal fertilizer and in combination with H2H. Bars are standard error of measurement and asterisks indicate statistical differences P <0.05.
[00158] [00158] Figure 15 shows a bar chart of the average height of the tomato after 30 days in soil treated with different combinations of organic fertilizers and H2H. Bars are standard measurement errors. Bo + H2H = bone meal with H2H. B1 + H2H = blood meal with H2H. Fea + H2H = feather meal with H2H.
[00159] [00159] Figure 16 shows a bar graph of the average dry weight of the biomass of tomato leaves and stems after 30 days in soil treated with different combinations of organic fertilizers and H2H. Bars are standard measurement errors. Bo + H2H = bone meal with H2H. B1 + H2H = blood meal with H2H. Fea + H2H = feather meal with H2H.
[00160] [00160] Figure 17 shows a bar chart of comparative body weights of growth-ending pigs fed with an agricultural mixture from this development and feeds for soy flour control. Control bars (blue) are on the left for each day; the bars for pigs fed the revelation farm mix are shown on the right (orange).
[00161] [00161] Figure 18 shows a bar graph of the average daily weight gain of end-of-growth pigs fed with an agricultural mixture of this disclosure in comparison with the soy flour control diet. The control bars are on the left; the bar for pigs fed with the agricultural mixture of the revelation is on the right (orange).
[00162] [00162] Figure 19 shows a bar chart of pig body weights that were fed an agricultural mixture of this disclosure compared to the soy flour control diet. Control bars (blue) are on the left for each day; the bars for pigs fed the revelation farm mix are shown on the right (orange).
[00163] [00163] Figure 20 shows images of chicks representative of each feed cohort: Control, 50:50 and 75:25 (Control: Ag / bread mixture) after 11 days of feeding.
[00164] [00164] Figure 21 shows the average weights per chick for each cohort of feed per day. The 75:25 ration cohort consistently presented a higher consumption of bird feed per day.
[00165] [00165] Figure 22 shows the average chick weight for each cohort of feed per day in line format.
[00166] [00166] Figure 23 shows the average weight gained per feed cohort. The 75:25 cohort showed a consistently higher average weight gain than the other cohorts.
[00167] [00167] Figure 24 shows the average feed intake per bird for each cohort of feed. The 75:25 feed cohort had the highest feed intake per bird per day.
[00168] [00168] Figure 25 shows the average feed intake per bird in line format.
[00169] [00169] Figure 26 shows the feed conversion rate per bird for each food cohort. The 75:25 food cohort and control foods had higher food conversions than the 50:50 cohort. Feed conversions reached all cohorts after day 10 of feeding.
[00170] [00170] Figure 27 shows the feed conversion rate for each cohort in the line format.
[00171] [00171] Figure 28 shows the digestibility of each feed cohort. Diets with Ag / bread mixture showed higher digestibility than the control diets.
[00172] [00172] Figure 29 shows a graph of the accumulated marketable production of strawberries per harvest day for cohorts treated with Grower's Standard, Grower's Standard with basalt, Grower's Standard with basalt and H2H (an emulsified agricultural mixture produced by the methods described in this document ) and Grower's Standard with H2H. The harvests were from Ventura County, California, for experiments carried out in the growing season of
[00173] [00173] Figure 30 shows a graph of the average weight per marketable fruit of strawberry cohorts treated with the cultivator pattern, cultivator pattern with basalt, cultivator pattern with basalt and H2H (an emulsified agricultural mixture produced by the methods described in the present document) and cultivator standard with H2H.
[00174] [00174] Figure 31 shows a graph of the cumulative revenue differential of the strawberry cohorts pattern of the producer treated with Grower's Standard, Grower's Standard with basalt, Grower's Standard with basalt and H2H (an emulsified agricultural mixture produced by the methods described in the present document) and Grower's Standard with H2H. Revenue is calculated in dollars per acre, less gross costs, and excludes the cost of administering the test program.
[00175] [00175] Figure 32 shows the accumulated weight yield of marketable strawberries harvested for cohorts treated with Grower's Standard compared to mixtures of Grower's Standard with agricultural additives for crops subjected to high temperatures (above 32.22ºC) during the growing season. growth, demonstrating an improved yield for crops treated with formulations containing agricultural mixtures.
[00176] [00176] Figure 33 shows the relative cumulative revenue of marketable strawberries harvested for cohorts treated with the standard of the grower compared to mixtures of the standard of the grower with agricultural additions to crops stressed with high heat (above 32.22ºC) during the season of growth, demonstrating an improved yield of the cultures treated with the formulations containing agricultural mixture.
[00177] [00177] Figure 34 shows a photograph demonstrating, inter alia, the consistent sizing differences of lettuce cohorts treated with agricultural mixtures produced by the processes described in this document compared to conventional Grower's Standard fertilizer and compared to conventional hydrolyzate fertilizer of fish.
[00178] [00178] Figure 35 shows a photograph of the consistent amount of chlorophyll and color of the lettuce cohorts treated with agricultural mixtures produced by the processes described in this document in comparison with the conventional Grower's Standard fertilizer and compared with the conventional fertilizer with fish hydrolyzate.
[00179] [00179] Figure 36 shows table 3.
[00180] [00180] Figure 37 shows table 5.
[00181] [00181] Figure 38 shows table 6.
[00182] [00182] Figure 39 shows table 7.
[00183] [00183] Figure 40 shows table 8.
[00184] [00184] Figure 41 shows table 9.
[00185] [00185] Figure 42 shows table 10.
[00186] [00186] Figure 43A shows Table 12.
[00187] [00187] Figure 43B shows Table 12, continued.
[00188] [00188] Figure 44 shows table 13.
[00189] [00189] Figure 45 shows table 14.
[00190] [00190] Figure 46 shows table 15.
[00191] [00191] Figure 47 shows table 16.
[00192] [00192] Figure 48 shows table 17.
[00193] [00193] Figure 49 shows table 4.
[00194] [00194] As used in this document, the term "recyclable biological stream" refers to a recyclable stream selected from: recyclable fresh food, blood meal, bakery products, used poultry, bagasse, fruit and / or selected vegetables and their mixtures.
[00195] [00195] As used herein, the term "stroke sieve" refers to a sieve or mesh to separate pasteurized solids, which can be used to produce animal feed, from pasteurized liquid hydrolyzate, and can include a variety sifting techniques. In some embodiments, the stroke screen may be an 18-60 mesh pore mesh (a diameter of about 250 to about 1,000 microns). In some embodiments, the stroke sieve can be an 18 mesh sieve with 1,000 micron openings, 20 mesh sieve with 841 micron openings, 25 mesh sieve with 707 micron openings, 30 mesh sieve with 590- openings 595 micron, 35 mesh sieve with 500 micron openings, 40 mesh sieve with 400 micron openings, 45 mesh sieve with 354 micron openings, 50 mesh sieve with 297 micron openings or 60 mesh sieve with 60 mesh openings 250 micron or other coarse selection technologies available on the market. A stroke screen can have an opening of 250 microns or larger, or between two of the sizes mentioned. In some respects, the filter or mesh is made of metal, plastic, glass or ceramic. In some ways, plastic can be nylon.
[00196] [00196] As used in this document, the term "fine sieve" refers to a sieve or mesh with pores of about 35 to 400 meshes (a diameter of about 500 to 27 microns). The fine sieve serves to i) increase the surface area of the particle, thus increasing the effectiveness of the enzymes used to produce the hydrolyzate; ii) ensuring the ability of the pasteurized hydrolyzate to pass easily through the farmer's drip lines or other similar equipment; and iii) ensuring that the particle size is appropriate for metabolism by soil organisms, since the agricultural mixture is released in the root area. In some embodiments, the 30 mesh screen is a vibrating screen. This separates the hydrolyzate from particles too large to pass through the mesh, for example, particles with an average diameter greater than 590 microns. The hydrolyzate that passes through the first screen can then be further separated by filtration through a 200 mesh screen with an opening size of 74 microns.
[00197] [00197] As used in this document, the term "cultivator standard" refers to a fertilizer based on nitrate or ammonia and another fertilization regime with standardized nutritional requirements for a given crop, in current use by the producer.
[00198] [00198] As used in this document, the term "hydrolyzate" refers to a product of digestion of a recyclable biological stream selected with enzymes. The liquid may contain small particles and / or oil droplets, depending on the grinders used and the mesh screen used to separate larger particles from the hydrolyzate, as in the present document described.
[00199] [00199] As used in this document, the term "agricultural mixture" refers to the composition that comprises nutritional components released from one or more biological currents recyclable by digesting proteins, carbohydrates (such as sugars, starches and / or cellulosic materials) and / or fats and oils in said recyclable biological stream to produce a composition containing, for example, amino acids, simple sugars, fatty acids and minerals, wherein the composition produced by the process comprises at least about 90% by weight with respect to the weight of the current recyclable biological starting material.
[00200] [00200] As used in this document, the term "ground biological paste" refers to the mixture that is formed after the first milling step, which can be a mixture of particles and liquid.
[00201] [00201] As used in this document, the term "incubated ground biological paste" refers to the mixture that is incubated at elevated temperature formed after the first milling step, which can be a mixture of incubated biological particles and an incubated biological hydrolyzate.
[00202] [00202] As used herein, the term "incubated biological particles" refers to particles obtained from the separated biological paste that are separated from the incubated biological hydrolyzate.
[00203] [00203] As used in this document, the term "incubated biological hydrolyzate" refers to the liquid hydrolyzate in the ground biological paste that is separated from the incubated biological particles.
[00204] [00204] As used in this document, the term "combination of enzymes" refers to two or more selected enzymes added to the ground biological slurry, the processed biological hydrolyzate and / or the incubation mixture. Enzymes in an enzyme combination can be mixed prior to addition to the milled biological pulp, the processed biological hydrolyzate and / or the incubation mixture, or they can be added separately to the milled biological pulp, the processed biological hydrolyzate and / or the mixture of incubation.
[00205] [00205] As used in this document, the term "high shear mixer" refers to an apparatus that disperses or transports a phase or ingredient (liquid, solid or gas) to a main continuous phase (liquid), with which it would be normally immiscible.
[00206] [00206] As used in this document, the term "agitation" means a stirring action designed to increase collisions between enzyme molecules and food particles. In some embodiments, agitation is produced by rotating the mixing slides in the incubation vessel, at a rate of 1 to 104 s-1.
[00207] [00207] As used in this document, the term "shear" means a cutting action that reduces the size of food particles, increasing their surface area and, therefore, their interaction with enzyme molecules. In some embodiments, high shear is created by circulating the pulp through a high-speed mixer and high shear throughout digestion at rates in the range of 105-106 s-1 or more.
[00208] [00208] In some embodiments, this disclosure refers to a process described in figure 1. A first recyclable biological stream 101 is subjected to a grinding and shearing step to form a first biological paste 102. A second, third or more recyclable stream optional biological 103 is subjected to a grinding and shearing step to produce a second, third or more biological sludge 104. Multiple recyclable biological streams can be processed in parallel or in series and combined with any of the products described in this document. The first biological paste is then incubated with one or more selected enzymes at a temperature of 21.1ºC to 62.7ºC. The incubated paste is then pasteurized at a temperature above 71.11ºC to produce a biological paste comprising biological hydrolyzate and biological particles 105.
[00209] [00209] A portion or all of the biological paste comprising biological hydrolyzate and biological particles 105 may be subjected to an optional drying step to produce the dry solid biological paste 106. In some embodiments, an antioxidant and / or anti-caking agents 109 are added to the dry solid biological paste. The dry solid biological paste 106 is subjected to a grinding or granulation step to form a ground or granulated product
[00210] [00210] A portion or all of the biological pulp comprising biological hydrolyzate and biological particles 105 can be separated into a biological hydrolyzate 111 and into biological particles 110. In some embodiments, the biological particles 110 can be recycled into the biological paste comprising biological hydrolyzate and particles or 105 with the first biological paste 102 to be incubated and pasteurized again. In some embodiments, biological particles 110 can be dehydrated through a separation step to produce dehydrated biological particles 112 and a recycled fraction that can be added to biological hydrolyzate 111. In some embodiments, dehydrated biological particles 112 can be used as compound, source of biofuel or as Animal feed (IV) 113. In some embodiments, an antioxidant and / or anti-caking agents 109 are added to Animal Feed (IV) 113. In some embodiments, biological hydrolyzate 111 can be mixed with the paste that is subjected to the drying step to supplement the dry biological paste 106, wherein the dry biological paste 106 would have a lower relative particle content of the dilution of the added biological hydrolyzate 111.
[00211] [00211] In some embodiments, biological hydrolyzate 111 is subjected to a centrifugation step to reduce the fat (oils) content in the produced centrifuged biological hydrolyzate 114 and form separate centrifuged oil 115. Centrifuged oil 115 can be further separated into one stream of unusable oil for food 122 and in a stream of oil usable in food 123. The stream of unusable oil for food 122 can be used as a source of biofuel
[00212] [00212] In some embodiments, an antioxidant and / or anti-caking agents 109 can be added to any of the dry biological sludge, ground or granulated product, dehydrated biological particles, to any animal feed (I) - (VI) or any form dryness of the animal supplier of this disclosure.
[00213] [00213] The centrifuged biological hydrolyzate 114 can be subjected to a stabilization step by adding a stabilizer to produce a stabilized aqueous hydrolyzate 116. The stabilized aqueous hydrolyzate 116 can be emulsified to produce an emulsified agricultural mixture 117. In some embodiments, the agricultural mixture emulsified 117 can be mixed with an additive. The mixed additive can include or exclude a dispersant or a mineral. The mineral can be extracted from basalt. The combined emulsified agricultural mixture can be used as a fertilizer 118.
[00214] [00214] In some embodiments, the emulsified agricultural product 117 may be concentrated to form a first concentrated agricultural mixture 119. The first concentrated agricultural mixture 119 may be used as a fertilizer or animal feed 120 as animal feed (II).
[00215] [00215] In some embodiments, an optional additional biological recyclable stream 121 may be added to the stabilized aqueous hydrolyzate 116. The optional additional biological recyclable stream 121 may be a recyclable carbohydrate stream. In some embodiments, the recyclable chain of carbohydrates can be dried bread crumbs.
[00216] [00216] In some embodiments, an optional additional recyclable biological stream 121 can be mixed with the pulp that is subjected to the drying step to supplement dry biological pulp 106, wherein dry biological pulp 106 would have a higher content of components in the additional biological recyclable stream 121. In some embodiments, when the additional biological recyclable stream 121 added to the pulp that is subjected to the drying step is a recyclable carbohydrate stream, the carbohydrate content of the dry biological pulp 106 is increased.
[00217] [00217] The inventors have discovered that, by the selective addition of centrifuged oil 115, optional additional biological recyclable stream 121, biological hydrolyzate 111 and / or centrifuged biological hydrolyzate (reduced fat) 114 in the process steps that precede the formation of any of the forms of animal feed described in this document, the content of amino acids, solids, carbohydrates and proteins can be selectively obtained to produce an ideal animal feed with properties that standard forms of animal feed cannot exhibit.
[00218] [00218] As indicated in figure 7, the first biological particles 110 and the dry agricultural mixture 134 can be combined, optionally with bread crumbs, to produce animal feed such as Animal feed (V). As shown in figure 8, the first biological particles 110 and the dry agricultural mix 134 formed from the combination of a first agricultural mix 128 and a second, third or more optional agricultural mixes 132, can be combined to form Animal feed (VI ).
[00219] [00219] In some embodiments, any of the animal feeds described in this document can be combined, mixed, diluted, dissolved, ground or sprayed with any other animal feed described in this document. In some embodiments, antioxidant and / or anti-caking agents can be added to any of the animal feeds described in this document.
[00220] [00220] The following experiment demonstrates that agricultural mixtures can be processed for use as fertilizer, plant growth stimulator or for soil correction.
[00221] [00221] Recyclables from fresh recycled foods were collected in supermarkets. Fresh food recyclables came from the supermarkets' meat, fish, bakery and deli departments, and were collected by refrigerated trucks within 2 days of being removed from the supermarket shelf. The recyclable fresh food chain from the bakery has been isolated from other recyclable fresh food chains and has not been included in the recyclable fresh food chains used to make the agricultural mix for use as fertilizer, plant growth stimulator or soil correction. The fresh food recyclables collected were kept fresh by storage in separate and specialized containers, designed to keep the collected food fresh while waiting for collection. Fresh food collected at the supermarket was processed within 24 hours of arrival at the production facility.
[00222] [00222] The fresh food recyclables collected were weighed and registered separately as kilos of meat or products. After the material was weighed, it was emptied into a central hopper and ground into a paste of recyclable particles of fresh food using a rotary knife grinder with a pump head.
[00223] [00223] The grinder was pumping the paste of recyclable particles of fresh food into a coated digestion container, where it was continuously mixed. The incubation process by enzymatic digestion was carried out in this vessel for a total of 3 hours. The enzymes were introduced into the paste, and the material was continuously heated, mixed and ground to maximize the efficiency of the enzymes that act on the material.
[00224] [00224] More specifically, a first combination of enzymes comprising endocellulase, exocellulase and lipase was added to the fresh food recyclable paste with constant mixing and the temperature was increased to 37.7ºC for 30 minutes. A high in-line shear grinder on a recirculation line was then connected. The high shear grinder was a high shear mixer with a disintegrating head (high cut action in RPM). A second combination of enzymes comprising pectinase, protease and α-amylase was then added, with the protease added last, and the temperature increased to 54.4ºC for 1 ½ hours. After incubation, the incubated hydrolyzate was heated between 71,11-76,6ºC for about 30 minutes to pasteurize the hydrolyzate.
[00225] [00225] The pasteurized material was then separated using mesh screens. The hydrolyzate produced by the incubation was first separated using a 30 mesh vibrating screen with an opening of 590 μm. The hydrolyzate that passed through the first screen was further separated by filtration through a 200 mesh screen with an opening size of 74 μm.
[00226] [00226] The separated liquid hydrolyzate was then introduced into a tricanter centrifuge and separated into particles, fats and an aqueous phase. The isolated aqueous phase (comprising from about 0.1 to 2.0 weight percent fats) was then emulsified / homogenized using an ultra high shear grinder, which can be a high shear multistage mixer, to form a hydrolyzate emulsified. The emulsified hydrolyzate was pumped into the stabilization tank for final processing. The isolated fats were pumped into a separate storage tank for further processing of the fat. The isolated particles were dried at room temperature. The isolated particles were optionally pelleted for use as a separate product for soil correction.
[00227] [00227] The pasteurized or hydrolyzed aqueous hydrolyzate was stabilized by adding phosphoric acid to a pH of 2.8, and then 0.25% potassium sorbate was added to preserve the liquid in its pasteurized state and prevent microbial activity during storage. This material was then sampled and checked for pH and the presence of food pathogens. The selection of food pathogens required an incubation period of 24 h, so that the material was kept in the stabilization tank for 24 hours until cleaning of this verification. The emulsified hydrolyzate was then transferred to a storage tank.
[00228] [00228] After stabilization, the hydrolyzate was also tested in the laboratory, to ensure that the content is free of pathogens (including E. coli and salmonella), heavy metals and other materials unsuitable for use as fertilizer, plant growth stimulator or soil correction. The individual batches were mixed to ensure that the aqueous emulsified hydrolyzate composition was consistent. Example 2. Procedure for obtaining agricultural mixture for use as animal feed
[00229] [00229] The following experiment demonstrates that agricultural mixtures can be processed for use as animal feed.
[00230] [00230] Recyclables from fresh recycled foods were collected in supermarkets. Fresh food recyclables came from the supermarkets' meat, fish, bakery and deli departments, and were collected by refrigerated trucks within 2 days of being removed from the supermarket shelf. The recyclable fresh food chain from the bakery was isolated from other recyclable fresh food chains and was not included in the recyclable fresh food chain used to make the agricultural mix for use as a fertilizer, plant growth stimulator or soil correction. The fresh food recyclables collected were kept fresh by storage in separate and specialized containers, designed to keep the collected food fresh while waiting for collection. Fresh food collected at the supermarket was processed within 24 hours of arrival at the production facility.
[00231] [00231] The fresh food recyclables collected were weighed and registered separately as kilos of meat or products. After the material was weighed, it was emptied into a central hopper and ground into a paste of recyclable particles of fresh food using a rotary knife grinder with a pump head. The recyclable stream of fresh bread separately was processed separately using a rotary knife grinder separated into bread crumbs.
[00232] [00232] The grinder was pumping the paste of recyclable particles of fresh food into a coated digestion container, where it was continuously mixed. The incubation process by enzymatic digestion was carried out in this vessel for a total of 3 hours. The enzymes were introduced into the paste, and the material was continuously heated and subjected to constant agitation and shearing, to maximize the efficiency of the enzymes that act on the material.
[00233] [00233] More specifically, a first combination of enzymes comprising endocellulase, exocellulase and lipase was added to the fresh food recyclable paste with constant mixing and the temperature was increased to 37.7ºC for 30 minutes. A high in-line shear grinder on a recirculation line was then connected. The high shear grinder was a high shear mixer with a disintegrating head (high cut action in RPM). A second combination of enzymes comprising pectinase, protease and α-amylase was then added, with the protease added last, and the temperature increased to 54.4ºC for 1 ½ hours. In some embodiments, enzymes can be added simultaneously. After incubation, the incubated hydrolyzate was heated between 71.1-76.6ºC for about 30 minutes to pasteurize the hydrolyzate.
[00234] [00234] The pasteurized paste material was then used directly as animal feed after confirming that the paste was free of pathogens in the case of pig tests. In the chicken tests and in the current configuration of the invention, the paste is moved to a heated process tank, then fed into a drum dryer, ground into powder and, as needed: stabilized; granulated and added anti-caking agent.
[00235] [00235] In an optional embodiment, the pasteurized suspension material was dehydrated at room temperature to obtain a dry feed form. In an optional embodiment, the pasteurized suspension material was mixed with the isolated bread crumbs processed by the methods described above and granulated into a solid form of animal feed.
[00236] [00236] In an optional embodiment, the pasteurized paste material was then separated using mesh screens. The hydrolyzate produced by the incubation was first separated using a 30 mesh vibrating screen with an opening of 590 μm. The hydrolyzate that passed through the first sieve was further separated by filtration through a 200 mesh sieve with an opening size of 74 μm.
[00237] [00237] The separated liquid hydrolyzate was then introduced in a tricanter centrifuge and separated into particles, fats and an aqueous phase. The isolated aqueous phase (comprising from about 0.1 to 2.0 weight percent fats) was then emulsified / homogenized using an ultra high shear grinder, which can be a high shear multistage mixer, to form a hydrolyzate emulsified. The emulsified hydrolyzate was pumped into the stabilization tank for final processing. Isolated fats were pumped into a separate storage tank for further processing of fat. The isolated particles were dried at room temperature. The isolated particles were separated.
[00238] [00238] The pasteurized or hydrolyzed aqueous hydrolyzate was stabilized by the addition of 0.25% potassium sorbate to preserve the liquid in its pasteurized state and prevent microbial activity during storage. This material was then sampled and checked for pH and the presence of food pathogens. The selection of food pathogens required a 24-hour incubation period, so that the material was kept in the stabilization tank for 24 hours until this check was cleared. The emulsified hydrolyzate was then transferred to a storage tank.
[00239] [00239] After stabilization, pasteurized aqueous hydrolyzate was also tested in the laboratory, to ensure that the content was free of pathogens (including E. coli and salmonella), heavy metals and other materials unsuitable for use as fertilizer, growth stimulator of plants or soil correction. The individual batches were mixed to ensure that the aqueous emulsified hydrolyzate composition was consistent.
[00240] [00240] The pasteurized aqueous hydrolyzate was then dehydrated to produce a dry form of animal feed.
[00241] [00241] In some optional embodiments, the recyclable bakery chain has not been processed by the enzymatic digestion methods described in this document and has instead been dried and ground into bread crumbs. In some optional embodiments, bread crumbs have been combined by mixing, grinding or diluting the crumbs with the dry or liquid forms of the hydrolysates described in this document to produce an agricultural mixture for use as animal feed. Example 3. Protection against crop stress
[00242] [00242] The following experiment demonstrates that agricultural mixtures produced by the methods described in this document can be used to allow the irrigation of crops under high stress conditions. High stress cultivation conditions can include or exclude: water with high salinity, soils with high salinity, low nutrient content in the soil, low volume of soil microbes and high heating. High salinity water stress
[00243] [00243] A strawberry crop in Ventura County, California (United States) was divided into four separate sections, and each section was subjected to separate conditions for irrigation and fertilization. A first section was fertilized with Grower's Standard, a standard nitrate fertilizer to serve as a control, the composition of which is described in Table 1 below. This section was irrigated with non-saline water. A second section was fertilized with Grower's Standard and irrigated with 200 ppm NaCl. A third section was fertilized with the same amount of Grower's Standard and "Ή2Η", an agricultural mixture of this development produced from recyclable fresh food using the methods described in this document. This third section was irrigated with 200 ppm NaCl. This third section was presented with an aqueous solution of H2H at an amount of 5 gallons per acre. A fourth section was fertilized with Grower's Standard and "H2H" and was irrigated with 200 ppm NaCl. This fourth section was presented with an aqueous solution of H2H at an amount of 10 gallons per acre. Table 1. Composition Grower's Standard
[00244] [00244] The results are shown in Table 2 below: Table 2. Summary of the results of high-salinity irrigation using the agricultural mixtures in this disclosure Experiment: Measure of the buffering effect of H2H in 200 ppm NaCl in the irrigation water in strawberries # Results Protocol 1 Grower's Standard - without control salt 2 GS + 200 ppm NaCl salt reduces the yield by 50% 3 GS w / NaCl + H2H (5 g / a) comparable to GS / Without salt 4 GS w / NaCl + H2H (10 g / a) highest total production within the experiment
[00245] [00245] The results shown in figure 10 indicate that irrigation with high salinity water reduces the harvest yield by 50%, when the crop is fertilized with only the control fertilizer. The treatment with Grower's Standard without salt is represented by the square line (pink). The treatment with the culture standard + 200 ppm NaCl is represented by the line (purple)
[00246] [00246] Strawberry cohorts were administered with Grower's Standard (half the application rate of the control) with H2H (administered at a rate of 5 gal / acre per day of treatment) ("H2H-low"), Grower's Standard ( half the application rate as a control) with H2H (administered at a rate of 7.5 gal / acre per treatment day) ("H2H-medium") or Grower's Standard (half the application rate as a control) with H2H (administered at a rate of 10 gal / acre per treatment day) ("H2H-high") for crops exposed to high temperatures above 32.22ºC during the growing season.
[00247] [00247] As shown in figure 32, cohorts treated with H2H and Grower's Standard consistently yielded a higher cumulative harvest of fruit per day than cohorts treated with Grower's Standard alone. As shown in figure 33, cohorts treated with H2H and Grower's Standard consistently yielded a greater cumulative difference in yield per hectare compared to cohorts treated only with Grower's Standard. The results demonstrate that the agricultural mixtures described in this document can increase the crop yield when applied under conditions of high stress. Example 4. Analysis and verification of batch to batch consistency of agricultural additives
[00248] [00248] To demonstrate the consistency of the batch-to-batch agricultural mix manufactured by the processes described in this document, eleven separate batches were analyzed for their composition. Table 3 lists the approximate analyzes of the solid and liquid samples on the basis of dry matter (DM). The percentage of dry matter (DM%), the percentage of crude protein (CP%), the crude energy (GE), the weight of the ash after ash formation (% of ash), the composition of the ether extract hydrolyzed with acid ( AEE%), equivalent to the fat content, the fiber percentage and the nitrogen free extract (NFE), equivalent to the carbohydrate content, were all measured in percentage by weight (% by weight), both for liquid hydrolyzate and for the separated solids.
[00249] [00249] The intrabatelada CV (coefficient of variance) was less than 36% for all parameters, except for the percentage of crude fiber that has a high CV from the low values. All percentages listed in this document for compositional analysis are percent by weight. For liquid samples, DM% ranged from 16.9 to 25.3%, PC ranged from 19.18 to 25.3%, GE ranged from 5504 to 6564 kcal.kg, ash value ranged from 3 , 93 to 9.32%, the AEE varied from 25.81 to 41.14%, the crude fiber varied from 1.8 to 7.6%, and the NFE varied from 8.26 to 26.51%. For solid samples, DM% ranged from 26.1 to 32.7%, PC ranged from 17.3 to 21.8%, GE ranged from 4779 to 5288 kcal.kg, the ash value ranged from 6 , 05 to 16.42%, the AEE varied from 15.06 to 20.51%, the crude fiber varied from 9.3 to 16.7% and the NFE varied from 23.86 to 35.82%.
[00250] [00250] As shown in figure 49, Table 4 lists the weight concentration of amino acids in the solid (separate particles) and liquid (agricultural mixture) samples made by the processes described in this document.
[00251] [00251] The compositional analysis of liquid and solid compositions indicates that each comprises nutrients that can be used to promote biological growth, including the growth of nematodes to increase crop yields and animal feed.
[00252] [00252] Tables 5 and 6 list the mineral content of agricultural mixtures produced by the processes described in this document. It was found that the intrabatelada CV was less than 16.4%, indicating a very consistent mineral content from batch to batch. For liquid samples, the weight percentage of calcium varied from 0.39 to 0.64%, phosphorus varied from 0.26 to 0.4%, potassium varied from 0.93 to 1.35%), magnesium ranged from 0.08 to 0.11% and sodium ranged from 0.37 to 0.58%. For liquid samples, the concentrations (in ppm, parts per million) of copper ranged from 3 to 5 ppm, of iron ranged from 92 to 133 ppm, of zinc ranged from 19 to 32 ppm and of manganese from 7 to 13 ppm. For solid samples, the weight percentage of calcium varied from 1.31 to 5.2%, phosphorus varied from 0.63 to 2.17%, potassium varied from 0.77 to 1.09%, magnesium ranged from 0.09 to 0.13% and sodium ranged from 0.33 to 0.61%. For solid samples, the concentrations (in ppm, parts per million) of copper ranged from 5 to 10 ppm, of iron ranged from 92 to 214 ppm, of zinc ranged from 49 to 79 ppm and of manganese ranged from 17 to 20 ppm .
[00253] [00253] Table 7 lists the carbohydrate content (% by weight) of agricultural mixtures obtained by the processes described in this document.
[00254] [00254] Tables 8, 9 and 10 list the saturated fatty acid content based on the percentage by weight (% by weight) of the total fat content (AEE%) on the dry matter basis of the agricultural mixtures made by the processes described in this document. With the exception of gonodic acid, it was found that the intrabatelado CV was less than 23.17%, indicating a very consistent batch to batch saturated fatty acid content. For liquid samples, the weight percentage of myristic (14: 0) ranged from 3.07 to 3.22%, from C15: 0 ranged from 0.41 to 0.48%, of palmitic (16: 0) varied from 26.24 to 27.25%, margarine (17: 0) ranged from 0.93 to 1.23%, from stearic (18: 0) ranged from 11.94 to 13.45%, from arachidic (20 : 0) ranged from 0.18 to 0.26%, from behenic (22: 0) ranged from 0.18 to 0.26%, from lignoceric (24: 0) ranged from 0.03 to 0.07%, myristoleic (9c-14: l) ranged from 0.5 to 0.75%, palmitoleic (9c-16: l) ranged from 3.31 to 3.90%, from 10c-17: l it was 0% , from elaidic (9t-18: l) ranged from 3.21 to 4.0%, from oleic (9c-18: l) ranged from 40.55 to 41.98% of vaccine (1 lc-18: l) ranged from 2.29 to 2.57%, from linoelaidic (18: 2t) ranged from 0.01 to 0.02%, from linoleic (18: 2n6) ranged from 10.14 to 14.53%, from linolenic ( 18: 3n3) ranged from 1.02 to 1.77%, from gonodic (20: ln9) ranged from 0.03 to 0.43%, from C20: 2 ranged from 0.16 to 0.19%, from homolinolytic (20: 3n3) ranged from 0.02 to 0.03%, from arachidonic (20: 4n6) ranged from 0. 23 to 0.28%, d EPA (22: ln9) ranged from 0.02 to 0.04%, clupanodonic (22: 5n3) ranged from 0.04 to 0.06%, DHA (22: 6n3) ranged from 0.07 to 0 , 11% and nervous (24:19) ranged from 0.01 to
[00255] [00255] The low CV indicates that the processes described in this document can produce agricultural mixtures with consistent composition profiles. Example 5. Mixing of basalt rocks with agricultural additives to obtain better crop yields
[00256] [00256] The basalt rock was combined with the agricultural mixtures described in this document after processing the agricultural mixture to produce an agricultural mixture enriched with minerals. Alternatively, the basalt rock was administered to the culture separately from the administration of the agricultural mixtures described in this document. The inventors surprisingly found that basalt rock comprises reduced iron (Fe (I) or Fe (II)) which can reduce organic compounds in the agricultural mixtures described in this document for ammonia gas (NH3) or ammonium salts (NΗ4 +) resulting in fertilizer compositions with a high nitrogen content. The fertilizer compositions with high nitrogen content of the agricultural mixtures mixed with basalt described in this document produced an increase in microbial activity, thus stimulating the crop yield.
[00257] [00257] The strawberries (cv. Per tola) used for this experiment were grown in a conventional field in Oxnard, California. This test was configured as a completely randomized block test of an organic H2H 3-2-1 fertilizer rate alone and in combination with a previous application of basalt material and compared to basalt alone superimposed on a cultivator pattern compared to a standard of cultivator, with completely random data collection of four replications maintained during the growing season. All treatments received conventional applications of nitrogen fertilizers, phosphorus and potassium in the season. All H2H materials were applied to the producers' underground drip tape during the season. The basalt material was spread manually before bed formation. Basalt in basalt: The standard Grower's formulation was applied at a rate of 0.5 tonnes per acre. Basalt in basalt: The standard Grower's formulation: H2H was applied at a rate of 0.5 tonnes per acre. When H2H was applied, it was applied at a rate of 10 gallons per acre per treatment. All cohorts were treated to the same levels as Grower's Standard.
[00258] [00258] As shown in figure 29, H2H with basalt and standard grower's exhibited a marketable increase in production in trays calibrated per acre for all treatments for each collection day, both daily and cumulatively. Basalt treatments in combination with H2H 3-2-1 and basalt alone produced the most extrapolated crates of strawberries during the experimental period, with the highest number of crates on average for the harvest period of 2037 and 1942 crates per acre, in compared to Grower's Standard at 1778 crates per acre. A different perspective on how nominal production affected the return of the final producer is shown in figure 31, which shows the daily tradable returns based on the USDA Shipping Point Market Prices found at HTTP: \ marketnews. usda.gov/portal (from the 2017 growing season) for each harvest day. This data is represented as the net revenue after the removal of costs of approximately US $ 6.00 per tray (for example, costs attributable to labor, costs of cardboard and tray, transport to the refrigerator and refrigeration costs associated with the removal of strawberries). Based on these data, the seasonal increase accumulated numerically for return to the producer was observed by the use of basalt with H2H and Grower's Standard in the other treatments. Figure 29 shows the daily use of the market for berries harvested during the season, that is, the percentage of marketable berries in relation to the total weight of the harvested berries, with significant differences observed in all treatments above the standard grower's, with use in between 80.6% and 84.4%. The best use was observed in the treatments GS: H2H: basalt, for GS: basalt, for GS: H2H in decreasing order. Figure 30 shows the net differential in returns to the farm for each day of harvest for treatment programs above Grower's Standard, which in this case was quite different for all treatments with GS: H2H: basalt at $ 2427, GS: basalt at $ 1341 per acre, and GS: H2H going on at $ 667 per acre.
[00259] [00259] The results demonstrate that the use of agricultural mix products produced by the methods described in this document in conjunction with a Grower's Standard program adds value to the producer's production. The results also demonstrate the synergistic effects of how H2H products (agricultural mix) are compared to a basalt product and basalt in combination with H2H products. Only basalt provides a higher yield than H2H 3-2-1, and only when an agricultural mixture is combined with basalt does the additional yield of the fruit weight and the increase in revenue are observed.
[00260] [00260] In some embodiments, the amount of agricultural mixture in this document described is 5 to 50 weight percent of the resulting mixture, preferably about 10 weight percent, based on dry matter. In some modalities, the application rate of basalt rocks is 226.8 kg to 907.18 kg (500 to 2,000 pounds) of basalt per acre per growing season. In some modalities, the application rate of agricultural mixture is from 19 L to 378.5 L (5 gallons to 100 gallons) per ton of basalt rock powder, to be composted before the application of the mixed compound, to be applied annually on organic crops and / or alfalfa, hay or pasture. In some embodiments, the agricultural mixture is applied once, twice or three times per growing season. Basalt rock mixed with the agricultural mixtures described in this document can be certified for use in organic agriculture. Basalt rock combined with the agricultural mixtures described in this document is applied to pastures for organic dairy products. Basalt rocks combined with the agricultural mixtures described in this document are applied to natural pastures for the production of beef and chicken without confinement. The mineral-enriched agricultural mixture can increase crop yield or increase the volume of fodder (the volume of food available for animals that feed on natural pastures or pastures) in regenerative agriculture by 5 to 25%, including 5, 6, 7 , 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 percent. Example 6. Processing soybeans with lettuce to produce an organic mixture of nutrients with a high nitrogen content
[00261] [00261] Organic soy flour can be hydrolyzed in water (9: 1 water: soy, by weight) at a temperature range of 60 - 82.22ºC and adding an alpha amylase and a protease, under constant shear conditions, using the systems described in this document, at a pH of 4.5, for about 3 hours, to create a soy flour paste.
[00262] [00262] It was found that the application of labile forms of carbon, including agricultural mixtures made by the processes described in this document, stimulates microbial activity, resulting in rapid alteration of bone, blood and feather meal. It was found that the rapid change produced a faster mineralization of nitrogen compared to the absence of said agricultural mixtures. Faster nitrogen mineralization resulted in faster plant growth.
[00263] [00263] Although it is known that bone, blood and feather flours stimulate beneficial microbial microorganisms in the soil (Quilty J., et al., Soil Res., 49, 1- 26 (2011)), prior to this disclosure, no information was known about the combination of bone meal, blood or feathers with the agricultural mixtures described in this document with respect to the effects of the microbe on soil, chemistry and plant growth.
[00264] [00264] Two experiments were carried out, one with soil only to measure mineralization and one with tomato seedlings to measure the effects of plant growth. Three changes were evaluated: a bone meal mixture (Nature Safe ™ 7-12-0), a blood meal mixture (Nature Safe ™ 8-5-5) and a feather meal mixture (Nature Safe ™ 13 -0-0). Each correction was analyzed for the increase in the growth and mineralization rates of the plants, alone and in combination with an agricultural mixture made by the methods described in this document ("H2H"). Controls included H2H alone and water. The rates of change additions were adjusted to normalize nitrogen application in all cases. H2H was diluted 10: 1 in water and applied at a rate of 50 gallons per acre. There were eight soil treatment conditions, as follows:
[00265] [00265] Only bone meal.
[00266] [00266] Only feather meal.
[00267] [00267] Only blood meal.
[00268] [00268] Only water.
[00269] [00269] Bone meal + H2H.
[00270] [00270] Feather meal + H2H.
[00271] [00271] Blood meal + H2H.
[00272] [00272] Only H2H.
[00273] [00273] The NPK (nitrogen-phosphorus-potassium) content listed in the corrections, with normalized nitrogen content and the amount of normalized correction added per experiment (performed in a tube) are summarized in Table 11. NPK ratio Amount of correction amount of required correction added for 453.9 g per nitrogen experiment Flour 7-12-0 6.48 kg 88 mg bone Flour 8-5-5 5.66 kg 77 mg blood Flour 13-0-0 3.49 kg 47 mg penalty Table 11: Summary of soil correction test conditions
[00274] [00274] The soil was collected from an uncorrected irrigated soil, previously planted with almonds. The soil was carefully mixed by hand at the moisture levels of the field and stored in a cold chamber (4-6ºC) until the beginning of the experiments. Before the experiments, the moisture content of the soil was adjusted to 40% of the water holding capacity.
[00275] [00275] The bioassay chambers were prepared from PVC columns (31.5 cm long, 4 cm in diameter) and covered at one end with a 6 mm diameter hole, with a mesh covering the hole to avoid soil loss. The holes were equipped with a removable cover. The columns and the PVC mesh were thoroughly washed with water before the soil was introduced. The PVC columns were then allowed to drip dry. Each of the eight treatment conditions was replicated five times, for a total of experiments (with one tube per experiment, for 40 tubes). For each treatment, a batch of soil was prepared, mixed with the treatment condition and divided into individual chambers. H2H was applied at a rate of 50 gallons per acre, diluted 10: 1 in water, reduced to the surface of the chambers (12.6 cm2), so that 0.06 mL of H2H was applied in 0.6 mL of water per tube. All experiments were carried out at room temperature. The experiments were performed twice.
[00276] [00276] To measure the leachate from each chamber, 100 mL of ddH2O (double distilled water) was added on the day of application (day 1) and also on days 3, 7, 14, 28 and 2 months. For each measurement, the bottom of the columns was unobstructed and allowed to drain for 2 hours. The samples were stored in plastic scintillation bottles of 15 mL at -20ºC until analysis of inorganic nitrogen. Nitrate and ammonium concentrations were determined by colorimetric analysis and comparison with standard curves by well understood methods (Keeney et al., Nitrogen - inorganic forms. In AL Page (ed.), Methods of Soil Analysis, part 2. Agron. Monogr. , 2nd ed. ASA and SSSA, Madison, WI, pages 643-698, 1982).
[00277] [00277] Tomato seedlings of the "Rutgers" variety were planted in corrected soils as described above, with five replicates of each treatment combination in 10.16 cm diameter containers. The seedlings were kept on growth benches in the laboratory for four weeks at room temperature, after which the plant size metrics, including plant height, dry weight, length and root biomass, were measured. Leachate analysis results
[00278] [00278] The nitrate content of the leachate in all treatments started out high and decreased rapidly, but no striking differences were observed in the rate of decrease over time between treatments, although the corrections themselves tended to be slightly higher (figure 11). There were some differences between treatments for individual dates. For example, in the first experiment on day 3, bone meal alone had significantly higher ppm nitrate than H2H in combination with bone meal (P <0.01, t = -4.0, figure 12), a trend which was repeated until the 14th (P
[00279] [00279] Although the ammonium concentrations were lower than nitrate, reaching only about 5 ppm, they showed a greater variation between treatments. For example, bone meal in the H2H combination showed higher concentrations of NH4 + (ammonium) than bone meal alone, both on day 1 (P = 0.03) and on day 3 (P <0.01) in experiment # 2 (Figure 13). A similar trend was observed in the first experiment, except on day 7 (P = 0.09), although the trend was not as strong. The increases in ammonium observed with H2H and bone treatment coincided with a reduction in nitrate. H2H similarly increased ammonium leachate to alter feather meal (figure 14), with feathers in combination with H2H having higher ammonium concentrations than isolated feathers on day 14 (P = 0.05).
[00280] [00280] The rapid nitrogen mineralization of organic fertilizers (correction to H2H) was observed in the first two weeks, with mineralization after the slowest process. Without being limited by theory, the enzymatic hydrolysis of urea and simple proteins at the seams releases nitrogen in the soil.
[00281] [00281] The synergistic increases observed in ammonium concentrations from the combination of corrections with H2H suggest an increase in the activity of microorganisms, which is consistent with the hypothesis that H2H can increase the availability of nutrients simulating the soil food chain. The combination of low nitrate with higher ammonium in the soil treated with H2H may indicate that more mineralization was occurring due to ammonification (ammonium production) instead of nitrification (nitrate production). Improved plant growth results
[00282] [00282] After 30 days, the H2H and control treatments grew more (figure 15). The soil biomass above the plants treated with H2H was 49% higher than plants treated with bone meal, 80%) higher than plants treated with blood meal and 56% higher than plants treated with feather meal, while controls were 34 %, 62% and 40% higher, respectively. Surprisingly, it was found that the inhibitory effect of corrections on growth was overcome by H2H. All plants grew more in the H2H correction combination treatments, compared to the corrections alone. A similar trend was observed in the total biomass of plants above the ground (figure 16). Biomass and root length differed little between treatments, and the rate of root sprouting, a measure of the amount of energy the plant is allocating in the biomass below ground versus above ground, did not show any major differences.
[00283] [00283] Without being limited by theory, the decreased counterintuitive growth observed with H2H correction combination treatments compared to controls may have resulted from the breakdown of more labile forms of nitrogen in urea at the beginning of the experiment, inhibiting the growth of plants. Without being limited by theory, the release of ammonia from such corrections could have a temporary toxic effect on sensitive microbes, although the effect probably depends on the type of soil and the rate of application. This inhibition of microbes could have slowed the nitrification process, limiting the nitrogen available to plants, slowing their growth. All types of amendments, however, have been observed to produce increased plant growth when combined with H2H, compared to isolated amendments. Example 8. Use of agricultural additives as animal feed - measures of animal weight growth
[00284] [00284] The pigs at the end of growth were fed a solid diet of soy and corn flour ("solid diet") or started with a diet comprising a form of liquid paste exemplary of an agricultural mixture of this development, comprising liquids and particulate matter ("agricultural mix diet") before switching to the solid diet. It was observed that the liquid form of the agricultural mixture in this disclosure, made from streams of recyclable biological waste, could be used as a sufficient source of feed for pigs.
[00285] [00285] It was found that the compositional analyzes of the hydrolysates produced by the methods described in this document are very close to the ideal protein profile for growing pigs. As described in Example 4, the indispensable amino acid profiles of some modalities of the hydrolysates produced by the methods described in this document are consistent between batches. Dry and liquid (and mixed) hydrolysates have been found to provide a balanced amino acid profile for growing pigs with ideal growth and reduced nitrogen excretion. The reduced excretion of nitrogen provides a larger volume of pigs per unit area, because the high excretion of nitrogen pollutes runoff water, the quality of the surrounding air and the quality of the soil. In addition, it has been discovered that the hydrolysates of this disclosure include appropriate amounts of minerals and nutrients for use as animal feed, including calcium, phosphorus, copper, iron and manganese. In some embodiments, hydrolysates can be supplemented with other minerals when used as an exclusive source of animal feed, including or excluding calcium, phosphorus, zinc and arsenic. In addition, hydrolysates produced by the methods described in this document contain higher amounts of disaccharides and oligosaccharides, but less starch compared to corn. The results indicate that hydrolysates are expected to provide more energy as animal feed than corn, because it is known that the high content of starch and fiber reduces the digestibility of amino acids, energy and other nutrients (Zhang, W., and others , 2013. The effects of the level of dietary fiber on the digestibility of nutrients in growing pigs, J. Anim. Sci. Biotechnol. 4, 17).
[00286] [00286] In the pig growth termination experiment, 64 pigs were divided into the solid diet group or the agricultural mix diet group. The pigs were monitored in three phases - the first phase of pigs weighing 35 to 60 kg (2 weeks); the second phase of pigs weighing 60 to 90 kg (for two weeks) and the third phase of pigs weighing 90 to 120 kg. In the study of end-of-growth pigs, the pigs fed the mixed feed diet were switched to the solid diet during the third phase of the test - the 90 to 120 kg weight phase. The measurements were obtained, including growth performance, daily weight gain, feed intake, feed efficiency and carcass quality.
[00287] [00287] In the breeding pig experiment, 108 pigs were divided into two groups - one group received a corn meal and soy diet, while the other group received the farm mix diet during phase 1 (2 weeks) and then switched to solid diet in the second phase (2 weeks). The measures were obtained, including growth performance, daily weight gain, feed intake, feeding efficiency and frequency of diarrhea.
[00288] [00288] Figure 17 shows that the agricultural mixture produced from recyclable materials was a suitable feed for pigs that finished cultivation, generating weight gains similar to the corn and soy flour diet (weight gains that were not significantly different control animals for corn and soybeans). This demonstrates that the mixtures in this disclosure can be used to provide healthy and sustainably farmed cattle.
[00289] [00289] In addition, figure 18 shows that the hydrolyzate diet also produced approximately the same average daily weight gain for the cornmeal and soy diet.
[00290] [00290] Although pigs fed hydrolyzate had gained marginally less weight by the 28th, supplementation of the hydrolyzate as described in this document, for example, adding carbohydrates and / or dehydrating the hydrolyzate in a granulated product sold will increase weight gain in pigs fed hydrolyzate, compared to pigs fed traditional solid foods, such as corn / soy flour. The animals fed with the liquid mixtures had larger stomachs than the control animals, indicating that the calorie consumption of the liquid diet was limited by the size of the animals' stomach. The animals produced less manure and less diarrhea when fed with the pre-digested composition. In addition, feeding the pigs with nutrient-rich compositions from this disclosure produces pigs with leaner meat, reduced diarrhea and / or other health benefits, such as lower incidence of infections and / or diseases.
[00291] [00291] The results of the newborn pigs show that the hydrolyzate diet constituted an adequate diet, generating weight gains similar to those of the cornmeal and soy diet, as shown in figure 19.
[00292] [00292] In addition, pigs fed hydrolyzate had reduced levels of diarrhea. Thus, in some modalities, feeding animals, such as pigs, with the hydrolyzate mixture improves animal health.
[00293] [00293] In addition, it was discovered that the hydrolyzate comprised a high level of unsaturated fatty acids, which was included in the feed. Animals fed a diet comprising unsaturated fatty acids from the hydrolysates described in this document are expected to exhibit a high amount of unsaturated fatty acids after slaughter. In non-ruminant animals, the fatty acid profiles in the tissues reflect the fatty acid profiles in their food. Food enriched with unsaturated fatty acids can, in some ways, increase the concentration of unsaturated fatty acids in pork (See Nguyen, LQ et al., Mathematical relationships between the intake of n-6 and n- 3 polyunsaturated fatty acids and their contents in adipose tissue of growing pigs, Meat Sci. 65, 1399-1406 (2003); Mitchaothai, J. et al., Effect of dietary fat type on meat quality and fatty acid composition of various tissues in growing – finishing swine, Meat Sci. 76, 95-101 (2007)), indirectly improving the health of pork consumers.
[00294] [00294] In some embodiments, hydrolysates of different fat compositions can be supplied to animals at different stages of growth. In some embodiments, hydrolysates made with reduced levels of fat using the tricanter centrifuge according to the methods described in this document can be supplied to weaned pigs. Hydrolysates made with non-reduced fat levels, according to the methods described in this document, can be supplied to pigs in later stages of growth, preferably during the period of growth stop, to increase the proven energy density and palatability of the diet ( Kerr, BJ et al., Characteristics of lipids and their feeding value in swine diets, J. Anim. Sci. Biotechnol. 6, 30 (2015)).
[00295] [00295] In some embodiments, additional nutrients can be added to the hydrolyzate to increase the weight gain of the animals for use of the agricultural hydrolyzate as animal feed to personalize the balance of carbohydrates and sugar in the animal feed.
[00296] [00296] In some embodiments, additional carbohydrates can be added to the hydrolyzate. Carbohydrates can be supplied, for example, by adding baked products or hydrolyzed baked products. In some modalities, bread crumbs, soy flour, distillery grains and / or almond shells can be added to the hydrolyzate for use as food supplements. Distiller's grains can include or exclude: barley, corn, rice and hops. In some embodiments, the hydrolyzate can be in dehydrated (essentially dry) or liquid form when combined with the additional carbohydrate source. In some embodiments, a supplement comprising 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60% or 65%, or any range of carbohydrate percentages between any two of the percentages indicated may be added to agricultural additives. In some modalities, the agricultural mixture supplemented with carbohydrate can be dehydrated and sedimented. In some embodiments, particulates from biological recyclables, for example, particulates obtained by filtration of the hydrolyzate or the tricanter centrifuge, can be added to the hydrolyzate. In some embodiments, the particulate material may be rich in proteins.
[00297] [00297] In some modalities, agricultural mixtures fed to weaned pigs can be supplemented with particles with a high protein content, while the hydrolyzate fed to pigs that finish growing can be supplemented with carbohydrate. In some embodiments, the agricultural mixture supplied to weaned pigs or pigs at the end of growth can be supplemented with fats, for example saturated and / or unsaturated fats. Supplementation of the agricultural mixture with carbohydrates, fats or proteins includes any process that increases the percentage of carbohydrates or proteins in the hydrolyzate by more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 , 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 , 39 or 40% or by any percentage range between any two of the quoted percentages. Example 9. High conversion animal feed using liquid agricultural additives
[00298] [00298] The agricultural mixture produced by the methods described in this document comprising a constituent that can include or exclude: proteins and / or peptides, fats, fibers and carbohydrates, can be used as pre-digested raw material for animals. The inventors recognized that the pre-digested feedstock has a higher mass conversion rate of the feed to animal weight compared to a standard food product. The animal feed product comprises undigested corn, soy, alfalfa and / or oats.
[00299] [00299] The agricultural mixtures in this disclosure can be used as feed for animals with a high conversion rate. Animals (pigs and / or chickens that are usually fed a diet of corn and soy flour) can be fed with the liquid or dry agricultural mixtures of this disclosure to gain weight with greater efficiency in the use of food (that is, a greater conversion rate of food to animals weight). In some ways, animals produce less manure and less diarrhea when fed with the pre-digested composition. Therefore, approximately 100% of the recyclable biological stream processed according to the methods of this disclosure can be used efficiently. Example 10. High conversion poultry feed using dry agricultural additives
[00300] [00300] The agricultural mixture produced by the described methods was used to feed chicks to demonstrate the improved conversion rate of the agricultural mixture, in relation to a control diet comprising soy and corn flour.
[00301] [00301] The control diet met or exceeded the Cobb recommendations for chicks. The control diet ingredients are listed in Table 14, assuming 90% dry weight. The composition of the control diet is listed in Table 15.
[00302] [00302] The control diet was mixed with the agricultural mixture ("H2H") and bread in the proportions by weight of
[00303] [00303] Three cohorts comprising 144 hatching chicks (broilers) per cohort were fed a 50:50, 75:25 diet, or with strict control over the first 14 days. The animals were allowed to eat ad libitum. The chicks were divided into six per cage, with 72 cages in total. One chick from each cage was sampled on days 6, 10 and 14, to determine the effects of food diets on chick growth and feed uptake. Representative sizes of the dietary treatment cohorts at 11 days of feeding are shown in figure 20. Figure 20 shows that the cohorts fed the 75: 25 (Control: Ag / bread mixture) achieved the highest overall volume of animals and meat cutting. Figure 21 shows that cohorts fed the 75:25 diet had the highest weight per bird ("treatment weights"). Figure 22 shows that the average weight of the cohorts fed 75:25 was consistently higher than that of the cohort fed the Control feed or the 50:50 feed. Figure 23 shows that the cohort fed with the 75:25 ration exhibited the greatest weight gain compared to the Control feed or the 50:50 ration. One of the reasons why the cohort fed with the 75:25 ration gained more weight was that this cohort consistently had the highest update per ration per bird (figure 24 and figure 25). The difference in feed conversion rate, however, was less pronounced between 75:25 and Control foods,
[00304] [00304] The serum chemistry of the sacrificed cohorts was analyzed, as shown in Table 17. The results indicate that the cohorts treated with mixtures of Ag and bread exhibited higher levels of cholesterol than the Control ration cohort, but lower glucose content and triglycerides after 14 days of feeding.
[00305] [00305] The results indicate that an adequate balance between fat content and pH in feed differences probably led to increased food uptake, which, when combined with the higher feed conversion rate of feeds with Ag / bread, led to the observed weight gain. Thus, the inventors demonstrated that the control of the composition of animal feed, such as Animal feed (I), produced by the methods described in this document, including removal or selective addition of fats, allows the production of an animal feed, which results in a surprisingly large difference in animal weight compared to animals fed a control diet. Example 11. Agricultural additives from Brassica as a natural pesticide
[00306] [00306] The liquid hydrolyzate obtained from the agricultural mixtures described in this document is useful to suppress or inhibit the growth of pests in the soil. Raw materials comprising brassica spp. produce high levels of isothiocyanate inhibiting soil pests from the hydrolysis of glycosinolates present in brassica spp. Glycosinolates are derived from amino acids and are stored in the vacuoles of cells of all types of tissues within the plant (M. Morra, et al., Soil Biology and Biochemistry, 2002, 34: 1683-1690). After tissue damage resulting from the milling, shearing and cellulase activity induced by the processes and enzymes described in this document, glycosinolates are cleaved by the addition of thioglycosidase (myrosinase; EC 3.2.1.1), producing many products, including isothiocyanates, nitriles and thiocyanates. Isothiocyanates are biologically active, disrupting cellular components, including those of soil pests by denaturing the protein structure.
[00307] [00307] A raw material comprising Brassica juncea (mustard green) is processed using the methods described in this document, where the processing enzymes include a cellulase to break down cell structure and, optionally, a thioglycosidase to maximize glycosinolate hydrolysis, which results in the release of isothiocyanate.
[00308] [00308] In some embodiments, the raw material may comprise one or more brassica species, including those described in this document.
[00309] [00309] Soil that has not been used in a growing season is divided into three or more parts. A portion is treated with water as a control. Another part of the soil is treated with inorganic fertilizer (Grower's Standard) as another control. Another part of the soil is treated with the agricultural mixture of raw material brassica spp. Another part of the soil is treated with the agricultural mixture of raw material of brassica spp. combined with inorganic fertilizer (Grower standard). Each part of the soil can be made in soil or in replicas. Tomato seeds (cv. Rhodade) are added to each part of the soil. A measured quantity of P. neglectus nematodes is then added to each part of the soil. The levels of soil nematodes are measured using the Baermann funnel method. General agronomic practices are implemented to grow the seedlings. Each soil sample with tomato seedlings is treated separately with water, water with inorganic fertilizer, water with agricultural mixture of raw material from brassica spp. and inorganic fertilizer and water with agricultural mixture of raw material from brassica spp. Nematode populations are monitored before nematode introduction, 1 day after nematode introduction, 2 days after nematode introduction, 3 days after nematode introduction, 1 week after nematode introduction and 2 weeks after nematode introduction nematode. Nematode populations of soil pests may decrease in soil samples treated with agricultural additives treated with raw materials from brassica spp. Example 12, Centrifugal processing of agricultural additives to separate agricultural additives into higher value product streams
[00310] [00310] The processes for obtaining the agricultural mixtures described in this document also include the use of centrifugal processing to separate the hydrolyzed paste into streams of higher value products. The hydrolyzate pastes prepared from fresh food streams were separated into aqueous, fat and solid phases using a tricanter centrifuge (Flottwegg Separator (Germany)). The hydrolyzate pastes tested showed NPK levels of 1-0-0, 1-1-0 (produced with high fish content), 3-2-1 (produced with high fish content) and 1-1-0 produced with 34% red meat. The use of the tricanter centrifuge allowed the separation of the paste in an aqueous phase, an oily phase (fat) and a solid phase. The fat content was reduced from 6-12% (by weight) in the paste to 0.2-1.4% in the isolated aqueous phase using the tricanter centrifuge. In some embodiments, the isolated aqueous phase was able to be subsequently dehydrated by the methods described in this document. In some modalities, isolated fats were further separated into high-tier and low-tier fats.
[00311] [00311] The use of centrifugal processing allowed the control of the amounts of fats, dry matter, crude protein and ash in the separated products, as shown in Table 12.
[00312] [00312] Table 13 shows the mass percentage variation of the separated aqueous phase composition compared to the paste after isolation using centrifugal processing. Example 13. Improvement of harvest quality
[00313] [00313] An emulsified agricultural mixture was prepared as described in this document. The mixture was reduced in fat content to less than 1.5% using the tricanter centrifuge. The combination was mixed with a dispersant to allow easy emulsification and release through drip pipe irrigation.
[00314] [00314] Nine unique replicas of romaine lettuce plants (cv. Green towers) were transplanted as buffers in a culture medium without unfertilized soil. The cohorts were soaked three days after transplantation and again two weeks later with 37.8 L (10 gallons) per acre of H2H 3-2-1 and an organic fish hydrolyzate fertilizer.
[00315] [00315] As shown in figure 34, lettuce 4 weeks after transplantation treated with H2H was consistently larger and greener than cohorts treated without fertilizer or fertilizer with fish hydrolyzate. As shown in figure 35, the cohorts treated with H2H exhibited a higher color (average of 4.9), measured on a color scale from 0 to 5 (with 0 the lowest and 5 the highest) and also exhibited a higher chlorophyll content (relative chlorophyll content, as analyzed with a Minolta SPAD meter) of 46.8, compared to no fertilizer (3.0 and 39.4, respectively) or fish hydrolyzate (4.3 and 42.0 , respectively). The results clearly demonstrate that the emulsified agricultural mixture with customized properties exhibits a significant difference in crop size and quality, compared to a standard inorganic fertilizer or conventional fertilizer with fish hydrolyzate.
[00316] [00316] The inventions described and claimed in this document have many attributes and modalities including, but not limited to those established or described or referenced in this Detailed Disclosure. It is not intended to be comprehensive, and the inventions described and claimed in this document are not limited to the features or modalities identified in this Detailed Disclosure, included for illustrative purposes only and not for restriction. One skilled in the art will readily recognize that many of the components and parameters can be varied or modified to some extent or replaced by known equivalents without departing from the scope of the invention. It should be appreciated that such modifications and equivalents are incorporated into this document as if presented individually. The invention also includes all the steps, characteristics, compositions and compounds referred to or indicated in this specification, individually or collectively, and any and all combination of any two or more of said steps or characteristics.
[00317] [00317] All patents, publications, scientific articles, websites and other documents and materials referred to or mentioned in this document are indicative of the skill levels of those skilled in the art to which the invention refers, and each of these documents and materials referred to is incorporated in this document as a reference, to the same extent as if it had been incorporated as a reference in its entirety individually or established in this document in its entirety. Candidates reserve the right to physically incorporate into this specification any and all material and information from such patents, publications, scientific articles, websites, electronically available information and other materials or documents mentioned. The reference to any applications, patents and publications in this specification is not, and should not be taken as an acknowledgment or any form of suggestion that they constitute valid prior art or are part of common general knowledge in any country in the world.
[00318] [00318] The specific methods and compositions described in this document are representative of preferred embodiments and are exemplary and are not intended to be limitations on the scope of the invention. Other objects, aspects and modalities will occur to those skilled in the art after considering this specification and are included in the spirit of the invention, as defined by the scope of the claims. It will be readily apparent to one skilled in the art that various substitutions and modifications can be made to the invention disclosed in this document d without departing from the scope and spirit of the invention. The invention described illustratively in the present document can suitably be practiced in the absence of any element or elements, or limitations or limitations, which are not specifically disclosed, in the present document, as essential. Thus, for example, in each example of this document, in modalities or examples thereof, any of the terms "comprising", "consisting essentially of" and "consisting of" can be replaced by any of the other two terms in the specification. In addition, the terms "comprising", "including", containing ", etc. should be read expansively and without limitation. The methods and processes described in this document illustratively and appropriately can be practiced in different step orders, and that they are not necessarily restricted to the step orders indicated in this document or in the claims. Also, as used in this document and the appended claims, the singular forms "one, one", "o, a" include reference to the plural, unless the context clearly indicates otherwise. Under no circumstances may the Patent be construed as limited to the specific examples or modalities or methods disclosed in this document. Under no circumstances may the Patent be construed as limited by any statement made by any Examiner or any other employee or employee of the Patent and Trademark Office, unless such statement is specific and unqualified or reservation expressly adopted in writing by the Claimants. In addition, titles, headings or the like are provided to improve the reader's understanding of this document and should not be read as limiting the scope of this document. Any examples of aspects, modalities or components of the invention mentioned in this document are to be considered non-limiting.
[00319] [00319] The terms and expressions that were used are used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions to exclude any equivalent of the features shown and described or portions thereof, however it is recognized that various modifications are possible within the scope of the invention as claimed. Thus, it will be understood that, although this has been specifically disclosed by preferential modalities and optional features, the modification and variation of the concepts disclosed in this document can be used by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention, as defined by the appended claims.
[00320] [00320] The invention has been described broadly and generically in this document. Each of the narrower species and subgeneric groupings that fall under generic disclosure are also part of the invention. This includes the generic description of the invention with a negative condition or limitation, removing any object of its kind, regardless of whether the cut material is specifically mentioned in this document.
[00321] [00321] Other modalities are within the claims that follow. In addition, where features or aspects of the invention are described in terms of Markush groups, those skilled in the art will recognize that the invention is also described in terms of any individual element or subgroup of elements of the Markush group.

权利要求:
Claims (42)
[1]
1. A process for producing an agricultural mixture from a selected biological recyclable stream, the process comprising the steps of: (a) providing a recyclable biological stream using a collection system; (b) grinding the recyclable biological stream using a first grinder and optionally a second grinder to produce a biological soil paste; (c) adding one or more selected enzymes to said soil biological paste; (d) increasing the temperature of the first biological soil paste from room temperature to at least one temperature between about 35ºC and about 60ºC and incubating the first biological soil paste under constant agitation and shearing at two or more temperatures between about 35ºC and about 60 ° C, thus producing a first incubated biological paste comprising first incubated biological particles and a first incubated biological hydrolyzate; (e) pasteurization of the biological soil paste incubated to exterminate pathogens; wherein the method further comprises steps (f) and (g) or (h) (A) - (C); (f) separating the first incubated hydrolyzate into a first incubated biological hydrolyzate and the first biological particles incubated using one or a plurality of size-based separation methods; and (g) reducing the fat content of the first pasteurized incubated hydrolyzate by centrifugation to form a centrifuged biological hydrolyzate and centrifuged oil; or (h) alternatively, where when steps (f) and (g) are not performed, the method further comprises the steps of: (A) drying the first pasteurized and incubated biological paste to form a solid and dry biological paste ; (B) grinding the solid biological slurry to form a dry biological slurry powder or granulating the dry solid biological slurry to form dry biological slurry microspheres; and (C) optional combination or mixing of the dry, organic biological pulp or microsphere of dry biological pulp with a recyclable stream of carbohydrates to form the animal supplier (I).
[2]
Process according to claim 1, in which, when steps (f) and (g) are carried out, the process further comprising the steps of: (D) stabilizing the centrifuged biological hydrolyzate to form a stabilized aqueous hydrolyzate; and (E) emulsifying the stabilized aqueous hydrolyzate to form an emulsified agricultural mixture and, optionally, adding a dispersant to the emulsified agricultural mixture.
[3]
A process according to claim 2, further comprising the step of concentrating the emulsified agricultural mixture.
[4]
Process according to claim 2, further comprising the step of combining the emulsified agricultural mixture with an additive.
[5]
5. Process according to claim 2, in which the dispersant is added and the dispersant is a surface active agent selected from: ACCELL CLEAN® DWD (D-16), BIODISPERS (D-9), COREXIT® EC9500A (D -4), COREXIT® EC9500B (D-19), COREXIT® EC9527A (Dl), DISPERSIT SPC 1000TM (D-5), FFT-SOLUTION® (D-17), FINASOL® OSR 52 (D-11), JD -109 (D-6), JD-2000 ™ (D-7), MARE CLEAN 200 (D-3), MARINE D-BLUE CLEAN ™ (D-18), NEOS AB3000 (D-2), NOKOMIS 3- AA (D-14), NOKOMIS 3-F4 (D-8), SAF-RON GOLD (D-12), SEA BRAT # 4 (D-10), SEACARE ECOSPERSE 52, SEACARE EPA ZI-400 (D-13), ZI-400 OIL SPILL DISPERSANT, sodium dodecyl sulfate (sodium lauryl sulfate), Arkopal N-300 (C9H19C6H4O (CH2CH2O) 30H), Brij 30 (polyoxyethylated linear alcohol), Brij 35 (C12H25O (CH2CH2O) 23H), Brij 56 (C16H33O (CH2CH2O) 10H), Brij 58 (C16H33O (CH2CH2O) 20H), EGE Coco (ethyl glycoside), Genapol X-150 (C13H27O (CH2CH2O) 15H), Tergitol NP-10 (nonylphenoletoloxylate), Marlipal 013/90 (C13H27O (CH2CH2O) 9H), Pluronic PE6400 (HO (CH2CH2O) x (C2H4CH2O) 30 (CH2CH2O) 28-xH), Sapogenat T-300 (C4H9) 3CH6O2 30H), T-Maz 60K (ethoxylated sorbitan monostearate), T-Maz 20 (ethoxylated sorbitan monolaurate), Triton X-45 (C8H17C6H4O (CH2CH2O) 5H), Triton X-100 (C8H17C6H4 (OC2H4) 10OH), Triton X-102 (C8H17C6H4O (CH2CH2O) 12H), Triton X-114 (C8H17C6H4O (CH2CH2O) 7.5H), Triton X-165 (C8H17C6H4O (CH2CH2O) 16H), Tween 80 (C18H37- C6H9O5-OH) (OC2) Cocamidopropyl betaine, ethoxylated nonylphenol, diethanolamine, propylene glycol, oleic acid sorbitan monoester , coconut oil monoethanolamide, poly (ethylene glycol) monooleate, polyethoxylated tallow amine, methyl dipropylene glycol ether, polyethylene glycol alkyl ethers, octaethylene glycol monododecyl ether, pentaethylene glycol monododecyl ether, monododicyl ether, monododecyl ether, monododecyl ether glycosidic ethers, decylglycoside ether, octyl glycoside, polyethylene, polyethylene, alkylphenyl ethers, Nonoxynol-9, glycerol alkyl esters, glyceryl laurate, sorbitan polyoxyethylene glycol alkyl esters, sorbitan hydrochloride; cetrimonium (CTAB), cetylpyridinium chloride benzene chloride bicarbonate (BZT), dimethyldioctadecylammonium chloride, dioctadecyldimethylammonium bromide (DODAB), docusate (dioctyl sodium sulfosuccinate), perfluoroocutanyl sulfonate, perfluorooctane sulfonate sodium-alkyl, lauryl ammonium sulfate, sodium lauryl sulfate, and phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and their combinations.
[6]
A process according to claim 2, wherein the concentration of dispersant (% by weight) is selected from: 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3%, 4%, 5%, 6%, 7%, 8% and 9%.
[7]
A process according to claim 2, wherein the stabilization of the centrifuged biological hydrolyzate comprises the addition of a stabilizing agent selected from: inorganic acid, organic acid, organic preservative or inorganic preservative.
[8]
A process according to claim 2, wherein emulsification is achieved by emulsifying the stabilized aqueous hydrolyzate with a high shear mixer.
[9]
Process according to claim 1, in which when steps (e) and (f) are carried out, the process further comprises the step of separating the first biological particles incubated in dehydrated biological particles and a recycled liquid fraction.
[10]
The process of claim 9, wherein separation is achieved with the use of a screw press, belt filter or hydraulic press.
[11]
Process according to claim 1, wherein the first separated incubated biological particles are added to a second or more streams of recyclable biological waste that are processed in the steps of claim 1.
[12]
A process according to claim 9, wherein an additive is added to the dehydrated biological particles.
[13]
13. Process according to claim 12, in which the additive is selected from basalt, granite, glauconite (green sand) and biotite.
[14]
Process according to claim 1, in which steps (f) and (g) are not performed and steps (h) (A) - (C) are performed.
[15]
Process according to claim 1, in which when step (g) is carried out, the centrifuged biological hydrolyzate is added to the biological paste in a different batch when the process of claim 1 is carried out separately with steps (h) (A) - (C).
[16]
The process according to claim 1, in which, when step (g) is performed, the centrifuged oil is added to the dry biological paste in a different batch, when the process of claim 1 is carried out separately with the steps (h ) (A) - (C).
[17]
A process according to claim 1, wherein when step (g) is carried out, the centrifuged oil is further separated into oil usable for food and oil unusable for food.
[18]
A process according to claim 1, wherein the one or more size-based separation methods comprise the use of a coarse filter, a fine filter or both.
[19]
A process according to claim 1, which further comprises the step of adding an antioxidant, anti-caking agent or both, to the dry and solid biological paste, to the soil or granular product or to an animal feed (I) to ( SAW).
[20]
A process according to claim 2, further comprising adding a second or more recyclable biological streams to the stabilized aqueous hydrolyzate.
[21]
21. The method of claim 1, wherein steps (h) (A) - (B) are carried out, the method further comprising combining or mixing the dry biological paste powder or the dry biological paste microspheres with a stream recyclable carbohydrate to produce animal feed (I).
[22]
22. The process of claim 21, wherein the recyclable carbohydrate stream is selected from one of the following carbohydrate sources: bread crumbs, bakery waste, nut shells, almond shells, soy flour grains, bagasse and still.
[23]
23. Process comprising carrying out the steps according to claim 1 with a first biological hydrolyzate, carrying out the steps of claim 1 with a second biological hydrolyzate, further comprising combining the centrifuged biological hydrolyzate from the first biological hydrolyzate with the biological hydrolyzate centrifuged process of the second biological hydrolyzate.
[24]
24. Process comprising carrying out the steps according to claim 2 with a first biological hydrolyzate, carrying out the steps according to claim 2 with a second biological hydrolyzate, further comprising combining the agricultural mixture emulsified from the process from the first biological hydrolyzate with the agricultural mixture emulsified from the process from the second biological hydrolyzate.
[25]
25. The method of claim 3, wherein the concentration of the liquid agricultural mixture is carried out using a vibrating filter, vacuum drum, vacuum evaporator, drum dryer, spray dryer, paddle dryer, rotary dryer or extruder.
[26]
26. The process of claim 1, further comprising the step of: (g) adding amino acids to the resulting products.
[27]
27. Centrifuged biological hydrolyzate produced by the process according to claim 1.
[28]
28. Centrifuged oil produced by the process of claim 1.
[29]
29. Dry biological mud granules, produced by any of the processes of claims 1, 14-16, 19-21 or 22.
[30]
30. Animal feed (I) produced by the process according to claim 21 or claim
22.
[31]
31. Emulsified agricultural additive produced by the process according to claim 2.
[32]
32. Dehydrated biological particles formed by the process according to claim 10.
[33]
33. Concentrated emulsified agricultural additive produced by the process according to claim 3.
[34]
34. Process according to claim 1, wherein the enzyme is selected from: at least one enzyme to digest proteins, at least one enzyme to digest fats and lipids and at least one enzyme to digest cellulosic material or at least one enzyme to digest other carbohydrates.
[35]
35. The process of claim 34, wherein the enzyme is selected from the group consisting of: xylanase, asparaginase, cellulase, hemicellulase, glumaiasis, beta-glumaiasis (endo-1,3, (4) -), urease, protease, lipase, amylase phytase, phosphatase, aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, alpha-galactosidase, beta-
galactosidase, glucoamidase, alpha-amylase, alpha-amylase invertase, laccase, mannosidase, oxidase, glucose oxidase, pectinolytic enzyme, pectinesterase, peptidoglutaminase, peroxidase, polyphenoloxidase, proteolytic enzyme, protease, ribonuclease and transglycoside
[36]
36. Process according to claim 1, in which the recyclable biological stream is selected from: blood or blood meal, bone or bone meal, feather or feather meal, manure, selected recyclable vegetables or fruits, vegetables that contain oils, grape marc, tomato pomace, olive pomace, fresh food recyclables, fish recyclables, carbohydrate recyclables, bread crumbs, bakery waste, nut shells, almond shells, pistachio shells, flour soy, bagasse and distillery grains and bakery recyclables.
[37]
37. Agricultural mixture produced by grinding a recyclable biological stream to produce a biological soil paste, heating and incubating the soil biological paste with one or more selected enzymes with constant agitation and shear, pasteurizing the incubated mixture to produce a biological hydrolyzate, reducing the fat content in the aqueous phase of biological hydrolyzate and stabilizing the aqueous phase by adding a stabilizer selected from an inorganic acid, an organic acid, an inorganic preservative or an organic preservative, to produce a stabilized agricultural mixture.
[38]
38. Agricultural additive according to claim 37, wherein the additive is dehydrated.
[39]
39. Method for increasing crop yield compared to a nitrate fertilizer, the method comprising: (a) providing the centrifuged biological hydrolyzate of claim 27, or the emulsified agricultural mixture according to any of claims 2, 5, 6 or 9-13; (b) contact of the agricultural mixture with a plant or plant component; (c) periodic water administration to crops; and (d) exposure of the cultures to air and a light source for a period of more than two weeks.
[40]
40. The method of claim 39, wherein the yield of the crop is increased by more than 10% over a nitrate fertilizer alone.
[41]
41. The method of claim 39, wherein the crops are subject to a high stress condition selected from: high salinity soil, high salinity water, low soil organic content, high temperature (greater than 32, 2ºC) and soils comprising low levels of micronutrients.
[42]
42. Method for increasing the weight of the animal or increasing the conversion rate of the feed to animal weight, the method comprising providing the animal with a formulation comprising a feed selected from feed (I) to feed (VI) ).

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同族专利:

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公开号 | 公开日

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WO2019033124A1|2019-02-14|

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JP2022009821A|2022-01-14|

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EP3665140A4|2021-06-02|

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CL2020000345A1|2020-06-19|

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EP3665140A1|2020-06-17|

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AU2018314285A1|2020-02-13|

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JP2020530295A|2020-10-22|

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US20190048307A1|2019-02-14|

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AR112971A1|2020-01-15|

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KR20200031122A|2020-03-23|

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AU2018314285B2|2021-07-08|

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CA3069807A1|2019-02-14|

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法律状态:
2021-09-08| B15K| Others concerning applications: alteration of classification|Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C05F 11/00 , C05F 17/00 , C05F 17/02 , A23K 10/37 Ipc: A23K 10/14 (2016.01), A23K 10/37 (2016.01), A23K 1 |

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2021-09-28| B07A| Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]|

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2021-11-03| B350| Update of information on the portal [chapter 15.35 patent gazette]|

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2022-01-25| B06A| Patent application procedure suspended [chapter 6.1 patent gazette]|

优先权:

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申请号 | 申请日 | 专利标题

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US201762544579P| true| 2017-08-11|2017-08-11|

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US62/544,579|2017-08-11|

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PCT/US2018/046571|WO2019033124A1|2017-08-11|2018-08-13|Agricultural admixtures|

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